Ada蛋白质在突变alkA基因启动子转录中的作用研究  

The Study on the Transcriptional Regulation of Mutant alkA Gene Promoter by Ada ProteinYu Chengguo, Xu Yi, Zhai Ling, et al

作　　者：于成国[1] 许懿[1] 翟玲[1] 苏荣健[1] 闻晓非 

机构地区：[1]中国医科大学基础医学院同位素中心

出　　处：《中国医科大学学报》1999年第3期169-171,共3页Journal of China Medical University

摘　　要：目的：研究Ａｄａ蛋白质对ａｌｋＡ基因表达的调节，探讨Ａｄａ蛋白质与ａｌｋＡ基因有效转录的关系。方法：采用ＤＮＡ顺序测定和体外转录的方法，以ｌａｃ基因启动子为对照进行实验。结果：Ａｄａ蛋白质加入以突变和野生ａｌｋＡ基因启动子为模板的体外转录体系中，产生的ａｌｋＡｍＲＮＡ高于无Ａｄａ蛋白质组；Ａｄａ蛋白质加入ｌａｃ基因启动子转录体系中，产生的ｌａｃｍＲＮＡ与未加Ａｄａ蛋白质组基本相同。结论：Ａｄａ蛋白质对ｌａｃ基因启动子转录调节作用不明显，对于突变的和野生的ａｌｋＡ基因转录均有促进作用，这种调节作用可能发生在启动子上游区。Objective: We investigated the relationship between Ada protein and the effective transcription of alkA gene in order to study the regulation of alkA gene expression by Ada protein. Methods: DNA sequencing and in vitro transcription had been used and the promoter of lac gene had been used as the control. Results: The amount of alkA mRNA transcripted from mutant and wild alkA gene promoters was higher in the group in presence of Ada protein than that in the group without Ada protein. There wasn't apparent difference between the two groups with and without Ada protein when lac gene promoter was used as template. Conclusion:T5BZThe transcription of lac gene promoter wasn't regulated by Ada protein,but the transcription of mutant and wild alkA gene promoters were effectively upregulated by Ada protein. This regulatory effect might be on the upstream of alkA gene promoter.

关 键 词：Ada蛋白质 alkA基因 启动子 基因转录 基因突变 

分 类 号：Q319.31[生物学—遗传学] Q754