腺苷A1受体激动剂延迟预处理对缺血再灌注兔心肌炎性细胞因子的影响  

Protection of adenosine A1 receptor agonist delayed preconditioning on rabbit myocardium after ischemic-reperfusion and influence to cytokine

作　　者：刘流[1] 冉珂[1] 常业恬[1] 

机构地区：[1]中南大学湘雅二医院麻醉科,湖南长沙410011

出　　处：《中国现代医学杂志》2010年第3期358-361,共4页China Journal of Modern Medicine

基　　金：湖南省自然科学基金资助项目(No:03JJY3053);湖南省科技厅资助项目(No:2007FJ3015)

摘　　要：目的探讨腺苷A1受体激动剂(2-氯环戊腺苷,CCPA)延迟预处理对兔心肌缺血再灌注损伤的保护机制及对炎性细胞因子的影响。方法30只健康新西兰雄性大白兔随机分成3组:假手术组(C组)、缺血再灌注组(I/R组)、CCPA组(P组),每组10只。C组仅行左冠脉套线而不阻断160min,I/R组行左冠脉阻断40min,再灌注120min,P组在静注CCPA0.1mg/kg24h后,处理同I/R组。各组分别于左冠前降支阻断前20min(T1)、左冠前降支阻断20min(T2)、左冠前降支阻断40min(T3)、心肌再灌注1h(T4)心肌再灌注2h(T5)5个时点抽取颈内动脉血测定血清中肌钙蛋白(cTnI)、白介素-10(IL-10)和白介素-6(IL-6)含量。再灌注结束后观察心肌细胞超微结构的变化,用伊文思蓝和TTC染色法测心梗面积。结果与C组比,I/R组与P组cTnI、IL-10和IL-6含量均增高(P<0.05),但与I/R组比,P组IL-10增高(P<0.05),心肌梗死面积减少(P<0.05),cTnI和IL-6降低(P<0.05)。结论腺苷A1受体激动剂延迟预处理通过调控炎性细胞因子平衡发挥心肌保护作用。【Objective】To investigate the protection of adenosine A1 receptor agonist（2-chloro-N6-cyclopenty-ladenosine,CCPA） delayed preconditioning on myocardial ischemia reperfusion injury and the changes of cytokine level in rabbits.【Methods】Thirty New Zealand male white rabbits were randomly assigned to 3 groups：Sham,I/R and CCPA group.Group CCPA was given CCPA 0.1 mg/kg before myocardial ischemia.Twenty-four hours later,I/R and CCPA underwent 40 min of coronary occlusion followed by 2 h of reperfusion.Blood samples were taken from arterial line at 20 min before occlusion（T1）,20 min after occlusion（T2）,40 min after occlusion（T3）,1 h after reper-fusion（T4） and 2 h after reperfusion（T5） for determination of plasma cTnI levels,IL-10 levels and TNF-αlevels.At the end of the reperfusion,infarct size（IS） and area at risk（AAR） were defined by Evans and TTC staining.The heart was harvested and ultrastructures were observed under the electron microscopy.【Results】The levels of cTnI in group CCPA was significantly lower than that of group I/R（P〈0.05）.CCPA significantly（P〈 0.05） reduced infarct size（22.1%±3.8% in group CCPA） of the left ventricular area at risk as compared with control（41.8±4.3% in group I/R）.The injury of group I/R was worse than that of group CCPA from the changes of the cellular structure un-der light microscope.Group CCPA had a lower levels of IL-6 and a higher level of IL-10.【Conclusion】CCPA pre-conditioning induces late cardio-protection against post-ischemic reperfusion injury in rabbits.

关 键 词：腺苷A1受体激动剂 延迟预处理 心肌 缺血再灌注 细胞因子 

分 类 号：R-332[医药卫生]