小儿急性淋巴细胞白血病p16和p15基因缺失与转录异常的研究  

Deletions and aberrant transcription of p16 and p15 genes in childhood acute lymphoblastic leukemia

作　　者：付国[1] 吕昌龙[1] 张瑞英[2] 胡宛如[2] 翟玲[1] 胡品良[1] 

机构地区：[1]中国医科大学免疫学教研室,沈阳110001 [2]中国医科大学附属第一临床学院

出　　处：《中华血液学杂志》1999年第7期366-368,共3页Chinese Journal of Hematology

摘　　要：目的　了解 p16 (MTS1)和 p15 (MTS2 )基因异常与小儿急性淋巴细胞白血病 (ALL)发生的关系。方法　用聚合酶链反应 (PCR)和Southernblot杂交法检测了 2 1例小儿ALL患者骨髓细胞 p16和 p15基因的缺失情况 ;用逆转录 聚合酶链反应 (RT PCR)技术检测了 19例患儿 p15mRNA表达。结果　经PCR检测 ,p16基因外显子 1,2均缺失者 8例 (38 1% ) ,p15基因外显子1,2均缺失者为 11例 (5 2 3% )。Southernblot检测的结果与PCR的结果相一致。RT PCR检测发现 2例 p15基因不缺失患儿的样品无p15mRNA表达。 结论　p16和p15基因在ALL患儿中存在高频率缺失和转录异常 。Objective To explore the role of p16(MTS 1) and p15(MTS 2) genes in the pathogenesis of childhood acute lymphoblastic leukemia(ALL) Methods PCR and Southern blot were used to analyse p16 and p15 gene deletions,RT PCR was used to analyse p15 gene transcription Results Exon l and 2 of p16 gene deletion was detected in 8 of 21 patients(38 1%), and exon l and 2 of p15 gene deletion in 11 of 21 patients(52 3%) Aberrant p15 gene transcription was observed in two cases Conclusion High frequency of p16 and p15 gene deletions and aberrant transcription may be involved in the pathogenesis of childhood ALL

关 键 词：白血病 ALL P16基因 P15基因 儿童 

分 类 号：R733.71[医药卫生—肿瘤]