酶法酯交换棉籽毛油合成生物柴油  

ENZYMATIC TRANSESTERIFICATION REACTION FROM CRUDE COTTON SEED OIL INTO BIODIESEL

作　　者：唐凤仙[1] 李春[2] 李元元[2] 赵来明[1] 徐小琳[1] 

机构地区：[1]新疆兵团化工绿色过程重点实验室/食品学院,石河子大学,石河子832000 [2]生命学院,北京理工大学,北京100081

出　　处：《太阳能学报》2010年第11期1397-1401,共5页Acta Energiae Solaris Sinica

基　　金：国家自然基金(20776017);北京市自然基金(5072028);石河子大学工科振兴计划专项基金(GKZX200503)

摘　　要：以戊二醛交联壳聚糖固定A.niger Li-38脂肪酶,固定条件为0.015%的戊二醛,交联50min。以该固定化酶催化棉籽毛油合成生物柴油,反应条件为:以叔丁醇为反应介质,醇油摩尔比1:1,水分含量0.6%,反应温度55℃,酶载量为8%,分批补加甲醇,反应28h后最终转化率达到89.1%。该固定化酶的贮藏稳定性较好,室温放置12d,酶活保持80%以上,30d时酶活为46.8%。固定化酶在30～70℃,pH值5.5～6.5之间较稳定,其热稳定性和pH稳定性较游离酶有所提高。固定化酶可重复使用7次,转化率保持在80%以上。Glutaraldehyde is chose to cross-bind Aspergillus niger Li-38 lipase on shell polysaccharide. The optimized condition is the concentration of glutaraldehyde 0. 015 % and reaction time 50min. The methanolysis of cottonseed biodiesel were carried out in tea-butanol solvent, with ration between methanol and oil 1： 1, water content 0.6%, under the temperature of 55q2, using immobilized enzyme of 8% （w/w）, and methanol adding in batch. The final conversion was 89.1% after 28h. The stability of immobilized lipase was improved. The activity has been kept up to 80% for 12d and 46.8% for 30d, storing in room temperature. It is stable at the range of temperature from 30 to 70℃, and pH 5.5 to 6.5. The immobilized lipase could be reused over 7 times, with the conversion ration over 80%.

关 键 词：固定化 生物柴油 棉籽毛油 脂肪酶 黑曲霉 

分 类 号：TQ517.4[化学工程]