树突状细胞联合重组腺病毒Ad-Mig基因治疗小鼠淋巴瘤的实验研究  

Combined dendritic cells and recombinant adenovirus-Mig gene therapy for murine lymphadenoma

作　　者：刁汇玲[1] 高金祥[2] 杨丽娟[1] 蒋淑君[1] 张建宾[3] 

机构地区：[1]滨州医学院生理教研室,滨州256603 [2]滨州医学院附属医院,滨州256603 [3]浙江大学免疫所,浙江杭州310058

出　　处：《中国现代医学杂志》2010年第6期824-827,共4页China Journal of Modern Medicine

摘　　要：目的观察树突状细胞(DC)和腺病毒介导的小鼠Mig基因对小鼠淋巴瘤的抗肿瘤效果。方法利用EG7细胞皮下注射C57BL/6小鼠建立小鼠淋巴瘤模型,单独或联合应用DC和携带Mig基因的重组腺病毒(Ad-Mig)直接进行瘤内注射治疗,观察小鼠皮下肿瘤生长情况。采用HE染色观察肿瘤组织的变化,用乳酸脱氢酶(LDH)释放法检测CTL和NK的杀伤活性。结果 DC能显著抑制荷瘤小鼠皮下肿瘤的生长,使肿瘤组织中炎细胞数量增多而肿瘤细胞数量明显减少,能显著增强荷瘤小鼠脾细胞NK和CTL杀伤活性。与单独应用DC相比,DC与Ad-Mig联合应用可明显提高抗肿瘤效果。结论 DC对小鼠淋巴瘤有显著治疗效果,且与Ad-Mig基因联合应用能进一步提高抗肿瘤效果。【Objective】To observe the antitumor effects of dendritic cell（DC） and recombinant adenovirus harbouring the murine Mig gene（Ad-Mig） on murine lymphadenoma in vivo.【Methods】C57BL/6 mice were inoculated subcutaneously with EG7 cells to establish the murine lymphadenoma model.The tumor-bearing mice were injected intratumorally with DC or DC combined with Ad-Mig respectively.Tumor growth was observed.The change of tumor mass was revealed by histological examination.The CTL and NK activity was measured in vitro by lactate dehydrogenase（LDH） release assay.【Results】DC could significantly inhibit the tumor growth,increase the number of inflammatory cells,and decrease the number of tumor cells.CTL and NK activity of the mice was also enhanced after treatment with DC.In contrast,co-delivery of DC and Ad-Mig led to stronger antitumor effects than DC alone.【Conclusion】Intratumoral injection of DC and Ad-Mig has significantly therapeutic effects on mice lymphadenoma.

关 键 词：树突状细胞 重组腺病毒Ad-Mig 细胞毒性T淋巴细胞 NK细胞 

分 类 号：R734.2[医药卫生—肿瘤]