PSD-93反义寡聚核苷酸鞘内注射对吗啡耐受大鼠的影响  

Effect of intrathecal PSD-93 AS ODN on rats with morphine tolerance

作　　者：张宇[1] 郭曲练[1] 王锷[1] 颜璐璐[2] 黄长盛[1] 邹望远[1] 

机构地区：[1]中南大学湘雅医院麻醉科,湖南长沙410008 [2]湖南省儿童医院麻醉科,湖南长沙410007

出　　处：《中国现代医学杂志》2010年第7期976-979,共4页China Journal of Modern Medicine

基　　金：国家自然科学基金项目30772080;国家自然科学基金项目30801074

摘　　要：目的观察鞘内注射PSD-93反义寡聚核苷酸(AS ODN)对慢性吗啡耐受大鼠的影响,并对其机制试做探讨。方法取鞘内置管成功的大鼠24只,随机分为4组(n=6),NS组予生理盐水20μL,每日1次。Mor组予吗啡40μg鞘内注射,每日2次以建立吗啡耐受模型。MA组予吗啡40μg鞘内注射,每日2次,同时予PSD-93AS DON 5μg鞘内注射,每日1次。MM组予吗啡40μg鞘内注射,每日2次,同时予PSD-93误义寡聚核苷酸(MS DON)5μg鞘内注射,每日1次,连续6d。于第6天注药后1h处死,取腰段脊髓用于nNOS及NMDAR1免疫组织化学检测。结果NS组、MA组NMDAR1、nNOS平均灰度均小于Mor组、MM组,NS组NMDAR1、nNOS平均灰度小于MA组。结论5μg PSD-93 AS ODN可减弱大鼠吗啡耐药现象。【Objective】To investigate the analgesic effect of PSD-93 AS ODN on rats with chronic morphine tolerance.【Methods】24 rats were successfully intrethecal intubation and randomly divided into 4 groups（n=6）.Group NS,20 μL NS a day intrethecal injection for 6 days.On the 6th day the rats were killed and spinal intrethecal injection;Group MM,5 μg PSD-93 MS ODN intrethecal injection;Group Mor,40 μg morphine intrethecal injection tiwce a day to set up the chronic morphine tolerance model;Group MA,5 μg PSD-93 AS ODN once a day and 40 μg morphine tiwce were removed for determination of nNOS and NMDAR1 expression by immunohistochemistry staining.【Results】The expression of NMDAR1,nNOS in group Mor and group MM were significantly higher than in group NS and group MA（P0.05）.The expression of NMDAR1,nNOS in group MA were significantly higher than in group NS（P0.05）.【Conclusion】5 μg PSD-93 AS ODN has effect on rats with morphine tolerance.

关 键 词：吗啡耐受 PSD-93蛋白 NMDA受体 NNOS 鞘内注射 

分 类 号：R614[医药卫生—麻醉学]