西乐葆对鼻咽癌CNE-1细胞增殖和凋亡的影响  

Effect of Celecoxib on The proliferation and apoptosis of CNE-1 cell

作　　者：戴幼艺[1] 刘保安[2] 段华新[3] 

机构地区：[1]中南大学湘雅医院肿瘤科,湖南长沙410008 [2]中南大学湘雅医院病理科,湖南长沙410008 [3]湖南省人民医院血液肿瘤科,湖南长沙410005

出　　处：《中国现代医学杂志》2010年第7期988-991,共4页China Journal of Modern Medicine

基　　金：湖南省自然科学基金(No:2004-2006;03jjy4028)

摘　　要：目的探讨鼻咽癌CNE-1细胞中环氧化酶-2(cyclooxygenase-2,COX-2)的表达及其选择性抑制剂通过抑制CNE-1细胞增殖和诱导其凋亡从而起抗肿瘤作用。方法细胞免疫化学法检测COX-2在鼻咽癌CNE-1细胞中的表达,对鼻咽癌CNE-1细胞以不同的西乐葆浓度0、1.25、2.50和5.00μM设A、B、C、D4个实验组药物干预,再分别行四唑盐(MTT)比色试验、克隆形成实验、流式细胞仪计数检测细胞的增殖能力、克隆形成能力及早、晚期凋亡4项指标。结果COX-2在CNE-1细胞内高表达;MTT法测得A组OD值与C、D组之间差异有显著性,A组与B组之间差异无显著性(P>0.05),B、C、D组两两比较差异有显著性,提示1.25μM西乐葆对CNE-1细胞增殖无影响,2.50～5.00μM西乐葆明显抑制CNE-1细胞增殖,且抑制作用随西乐葆剂量增加而增强,克隆形成及早、晚期凋亡率A组与B、C、D组比较差异均有显著性(各P<0.05),B组与C组差异无显著性(P>0.05),B组与D组之间差异有显著性(P<0.05);C、D组之间的差异无显著性(P>0.05)。提示西乐葆对CNE-1细胞的克隆形成能力有抑制作用,对CNE-1细胞早、晚期凋亡有明显的诱导作用,其作用与西乐葆浓度存在相关性,5.00μM较1.25μM西乐葆对CNE-1细胞增殖抑制和诱导凋亡作用明显增强,而在1.25μM与2.50μM间、2.50μM与5.00μM间比较,其对CNE-1细胞增殖抑制和诱导凋亡作用差异无显著性。结论①CNE-1鼻咽癌细胞为COX-2阳性表达细胞。②西乐葆对CNE-1细胞的增殖、克隆形成能力具有抑制作用,并能诱导早、晚期凋亡,从而对CNE-1细胞存在抗肿瘤作用。③西乐葆对CNE-1细胞的抗肿瘤作用与西乐葆浓度存在剂量相关性。【Objectives】To study whether cyclooxygenase-2（COX-2） is overexpressed in CNE-1 Cell.To evaluate whether the selective inhibitor of COX-2（a selective inhibitor of COX-2） can inhibit the proliferation and induce the apoptosis of CNE-1 cell（a human nasopharyngeal carcinoma cell line）.【Methods】 The protein expres-sion of COX-2 in CNE-2 cells was detected by immunohistochemical staining.The study was devived to four groups by the density of Celecoxib.The 0 μM,1.25 μM,2.50 μM,5.0 μM Celecoxib groups mean A,B,C,D group.MTT assay and colony forming assay were performed to determine the influence of Celecoxib on growth and proliferation of human nasopharyngeal cancer CNE-1 cells.Apoptosis of CNE-1 cells were determined by flow cytometry.【Result】The immunocytochemical staing showed that COX-2 was expressed in CNE-2 cell line cells.Celecoxib can inhibit the proliferation and colony forming and induce early and late stage apoptosis of CNE-1 cell line cells.There were differences in optical densities in MTT assay between group A and C and between group A and D,but no differences between group A and B.And there are differences between group B and C and between group C and D.These results indicate that Celecoxib has no effect on the proliferation of CNE-1 line cells at concentration 1.25 μM,but it has inhibitory effects at concentration of 2.50 μM and 5.00 μM,furthermore,inhibitory effects is more when the concentration of Celecoxib is larger.The numbers of colonies are lower and the early and late stage apoptosis cells are higher in Celecoxib alone group B,C and D,as compared with group A.Furthermore,there is difference in numbers of colonies and the early and late stage apoptosis cells between group B and D,but neither difference between group C and D nor between group B and D.【Conclusion】①CNE-1 cell line cells expressed COX-2.② Celecoxib can inhibit the proliferation and induce the apoptosis of CNE-1 cell line cells.③The anti-tumor effects of Celecoxib is more when the Celecoxib＇s c

关 键 词：鼻咽癌 增殖 凋亡 环氧化物酶 

分 类 号：R739.63[医药卫生—肿瘤]