Analysis of expressed sequence tags from the Ulva prolifera (Chlorophyta)  

作　　者：牛建峰 胡海岩 胡松年 王广策 彭光 孙松 

机构地区：[1]Key Laboratory of Experimental Marine Biology,Institute of Oceanology,Chinese Academy of Sciences [2]Key Laboratory of Genome Sciences and Information,Beijing Institute of Genomics,Chinese Academy of Sciences [3]Graduate University of Chinese Academy of Sciences [4]College of Marine Science and Engineering,Tianjin University of Science and Technology

出　　处：《Chinese Journal of Oceanology and Limnology》2010年第1期26-36,共11页中国海洋湖沼学报（英文版）

基　　金：Supported by the Scientific and Technical Supporting Programs of China (2008BAC49B01);the National Natural Science Foundation of China (No. 30830015)

摘　　要：In 2008, a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao. The causative species was determined to be Enteromorpha prolifera （Ulva prolifera O. F. Miiller）, a familiar green macroalga along the coastline of China. Rapid accumulation of a large biomass of floating U. prolifera prompted research on different aspects of this species. In this study, we constructed a nonnormalized cDNA library from the thalli of U. prolifera and acquired 10072 high-quality expressed sequence tags （ESTs）. These ESTs were assembled into 3 519 nonredundant gene groups, including 1 446 clusters and 2 073 singletons. After annotation with the nr database, a large number of genes were found to be related with chloroplast and ribosomal protein, GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy. In addition, rather comprehensive carbon fixation pathways were found in U. prolifera using KEGG. Some stress-related and signal transduction-related genes were also found in this study. All the evidences displayed that U. prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation. Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum （Ulvophyceae）.In 2008,a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao.The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O.F.Müller),a familiar green macroalga along the coastline of China.Rapid accumulation of a large biomass of floating U.prolifera prompted research on different aspects of this species.In this study,we constructed a nonnormalized cDNA library from the thalli of U.prolifera and acquired 10 072 high-quality expressed sequence tags (ESTs).These ESTs were assembled into 3 519 nonredundant gene groups,including 1 446 clusters and 2 073 singletons.After annotation with the nr database,a large number of genes were found to be related with chloroplast and ribosomal protein,GO functional classification showed 1 418 ESTs participated in photosynthesis and 1 359 ESTs were responsible for the generation of precursor metabolites and energy.In addition,rather comprehensive carbon fixation pathways were found in U.prolifera using KEGG.Some stress-related and signal transduction-related genes were also found in this study.All the evidences displayed that U.prolifera had substance and energy foundation for the intense photosynthesis and the rapid proliferation.Phylogenetic analysis of cytochrome c oxidase subunit I revealed that this green-tide causative species is most closely affiliated to Pseudendoclonium akinetum (Ulvophyceae).

关 键 词：green tide Ulva Prolifera expressed sequence tag （EST） filamentous green alga rapid growth PROLIFERATION 

分 类 号：S917.3[农业科学—水产科学]