厌氧纤维素降解产氢复合菌系L-3研究  

Cellulose Degradation and Hydrogen Production by Anaerobic Microbial Consortium L-3

作　　者：王佳婧[1] 刘晓风[1] 袁月祥[1] 闫志英[1] 廖银章[1] 郝纯[1] 贺蓉娜[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2010年第1期104-108,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家科技支撑项目(Nos.2006BAJ04B02;2006BAD07A02);国家"863"项目(No.2007AA100702);中国科学院知识创新工程重大项目(Nos.KSCXZ-YW-11)资助~~

摘　　要：从厌氧发酵污泥中筛选到一组高效、传代稳定的厌氧纤维素降解复合菌系L-3.该复合菌系的内切葡聚糖酶活(Cx)、滤纸酶活(FPA)、外切葡聚糖酶活(C1)、β-葡聚糖苷酶活(β-glucodase)分别为0.216、0.101、0.132、0.002U/mL;该复合菌系可使滤纸在42h内溃烂,并能在降解纤维素的同时产生氢气,气体中氢气含量最高可达70.2%,d13时滤纸失重率为70.6%.DGGE结果表明,该复合菌系主要由14种菌组成.在所选实验条件内,该复合菌系产纤维素降解酶的最适条件为:最佳碳源为滤纸,最佳氮源为硫酸铵,温度36℃,pH6.5～7.0,接种量5%.Microbial consortium L-3 was isolated from the anaerobic fermentation residue of corn stalk,and it could degrade cellulose and produce hydrogen.The Cx,FPA,C1 and β-glucosidase activity of consortium L-3 could reach 0.216 U/mL,0.101 U/mL,0.132 U/mL and 0.002 U/mL,respectively.In the filter degrading process,the filter paper collapsed in liquid culture within 42 h and the filter degrading rate could reach 70.6% on the thirteenth day.At the same time,hydrogen was produced and its highest content was 70.2%.DGGE results showed that consortium L-3 mainly consisted of 14 kinds of bacteria.In this experiment,filter was used as carbon source and（NH4）2SO4 as nitrogen source,and temperature 36 ℃,pH 6.5～7.0 and volume of inoculum 5% were applied for optimum conditions of cellulase production.

关 键 词：厌氧发酵 纤维素降解 产氢 复合菌系 纤维素降解酶 

分 类 号：TQ925[轻工技术与工程—发酵工程]