一株高浓度苯胺、苯酚降解菌的分离鉴定及降解特性  

Isolation and Characterization of an Aniline-degrading and Phenol-degrading Bacterium

作　　者：武洪杰[1] 谭周亮[1] 刘庆华[1] 李旭东[1] 

机构地区：[1]中国科学院成都生物研究所,成都610041

出　　处：《应用与环境生物学报》2010年第2期252-255,共4页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(No.50678165);国家"863"重大项目课题(No.2007AA06A409)资助~~

摘　　要：从某生活污水厂活性污泥中分离到一株能够以苯胺或苯酚为唯一碳源、能源生长的高效降解菌菌株ANP.经形态特征、生理生化及16S rDNA序列分析,将该菌株鉴定为Delftia sp.进一步研究表明,该菌株利用苯胺生长的最适温度和pH分别为30℃和6.0,最适降解浓度为2000mgL-1;利用苯酚生长的最适温度和pH分别为35℃和8.0,最适降解浓度为1500mgL-1.苯胺、苯酚混合培养时该菌株对苯酚的降解过程要滞后于对苯胺的降解过程,但经过42h均能彻底降解.研究了ANP降解苯胺和苯酚的开环途径,苯胺芳环通过间位途径裂解,苯酚芳环则是通过邻位途径裂解.A bacterial strain ANP capable of efficiently degrading aniline and phenol was isolated from activated sludge of a sewage treatment plant.The strain can be cultivated by using aniline and phenol as its sole carbon and energy sources.It is identified as Delftia sp.according to its morphology,biochemical properties and 16S rDNA sequence analysis.Further study indicated that the optimal pH and temperature for cell growth and aniline degradation were 6 and 30 ℃,respectively;the optimal concentration of aniline was 2 000 mg L^-1;the optimal pH and temperature for cell growth and phenol degradation were 8 and 35 ℃,respectively;and the optimal concentration of phenol was 1 500 mg L^-1.When cultivated with both phenol and aniline,strain ANP could only degrade aniline at first,but the both could be degraded thoroughly after 42 h.Enzymatic analysis showed that aniline was degraded in meta cleavage pathway,while phenol in ortho cleavage pathway.Fig 4,Tab 1,Ref 18

关 键 词：苯胺 苯酚 降解菌 Delftia sp. 邻苯二酚双加氧酶 

分 类 号：X172[环境科学与工程—环境科学]