甜荞·苦荞和大野荞的高分子量种子蛋白亚基研究  

Study on HMW Seed Protein Subunits in Common Buckwheat,Tartary Buckwheat and Fagopyrum megaspartanium Accessions

作　　者：王燕[1] 陈庆富[1] 

机构地区：[1]贵州师范大学生命科学学院植物遗传育种研究所,贵州贵阳550001

出　　处：《安徽农业科学》2010年第33期18678-18680,共3页Journal of Anhui Agricultural Sciences

基　　金：国家科技支撑计划(2006BAD02B06);国家自然科学基金(3106020);贵州省动植物育种专项(黔农育专字[2010]023号)

摘　　要：[目的]探讨甜荞、苦荞和大野荞的高分子量种子蛋白亚基。[方法]利用SDS-PAGE电泳法对18个不同地方居群的大野荞、3个甜荞和3个苦荞收集系的高分子量种子蛋白亚基进行研究。[结果]从这3个物种的24个收集系中,在高分子量区域（20-70 kDa）共发现12个不同的种子蛋白亚基谱带,种间差异大,种内差异小。3个物种在种子蛋白亚基分布上存在一定的相似性,约30%的谱带相同。其中,甜荞发现有9个谱带,含1个独特谱带（SSP33.71）;苦荞有8个谱带,含不同于甜荞的2个独特谱带（SSP39.15和SSP29.35）;大野荞有7个谱带,不仅含有苦荞的独特谱带,而且其谱带分布与苦荞极为相似。[结论]该研究为荞麦品质遗传育种、种间系统关系、高品质种子蛋白亚基基因工程等研究提供指导。[Objective] The study aimed to discuss the HMW seed protein subunits in common buckwheat,tartary buckwheat and Fagopyrum megaspartanium accessions.[Method] The HMW seed protein subunits of 18 wild buckwheat accessions of F.megaspartanium native to in different places and 6 common buckwheat and tartary buckwheat accessions were studied by means of SDS-PAGE.[Result] There were totally 12 seed protein subunit bands in high molecular weight region（20-70 kDa）discovered in 24 accessions from 3 species,with great variation among different species and little variation within the same species.3 species had similar spectrum of seed protein subunits and about 30% bands were same.Among them,The common buckwheat had 9 bands including a special band（SSP33.71） different from others.Tartary buckwheat had 8 bands including 2 special bands（SSP39.15 and SSP29.35） different from common buckwheat.F.megaspartanium had 7 bands,which not only include those special bands of tartary buckwheat,but also its band distribution was similar with that of tartary buckwheat.[Conclusion] The study provided the guidance for the studies on quality genetic breeding,interspecies system relations,gene engineering of buckwheat seed protein subunits.

关 键 词：甜荞 苦荞 大野荞 SDS—PAGE 种子蛋白亚基 

分 类 号：S517[农业科学—作物学]