基于银镜的高灵敏表面增强拉曼光谱基底制备及其用于呋喃唑酮检测  

Highly Sensitive Silver Mirror Substrate Preparation and Its Application to Surface Enhanced Raman Spectroscopic Detection of Furazolidone

作　　者：陆冬莲[1] 韩鹤友[1] 梁建功[1] 

机构地区：[1]华中农业大学理学院,农业微生物国家重点实验室,武汉430070

出　　处：《分析化学》2010年第5期715-718,共4页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20975042);湖北省自然科学基金重点项目(No.2008CDA080);武汉市青年科技晨光计划(No.200850731359);转基因科技重大专项(No.2009ZX08012-015B)资助

摘　　要：采用化学沉积法制备了表面增强拉曼散射光谱(SERS)银镜基底,用NaCl溶液与HCl溶液除去银镜表面杂质后,通过扫描电子显微镜对基底进行了表征,表明该基底表面的银纳米粒子平均粒径约为200nm,以对巯基苯胺为探针分子测得其增强因子为4.6×105。利用表面增强拉曼光谱及表面吸附选择定律研究了广谱抗菌药呋喃唑酮在该基底表面的吸附状态,证明呋喃唑酮分子主要是通过CN吸附于银纳米粒子表面的。A silver mirror reaction method was applied to deposit silver nanoparticles on a glass substrate. The effect of different reagents on these silver mirror substrates was investigated. SEM image for this silver mirror substrate shows that the average diameter of silver nanoparticles is about 200 nm. The surface-enhanced raman scattering（SERS） spectra of 4-aminothiophenol（PATP） molecule on this substrate were measured. The results indicated that the SERS-active silver mirror substrate made in this way has an enhancement factor of 4.6×105. These substrates were used to collect the surface-enhanced Raman spectra of furazolidone, which were widely used deleterious antibiotics. It was found that furazolidone might interact with silver surface through the lone pair electrons of the CN nitrogen atoms.

关 键 词：银镜 对巯基苯胺 呋喃唑酮 表面增强拉曼光谱 

分 类 号：TQ251.1[化学工程—有机化工]