类固醇类激素GC-MS分析中酮基衍生化技术研究进展  

Progress on Keto Groups Derivatization of Steroid Hormones in Gas Chromatography-Mass Spectrometric Analysis

作　　者：方锴[1] 潘学军[1] 黄斌[1] 刘晶靓[1] 王宇[1] 高建培[1] 

机构地区：[1]昆明理工大学环境科学与工程学院,昆明650093

出　　处：《分析化学》2010年第5期743-751,共9页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20767002);国家环保公益性行业科研专项基金(No.200809136);教育部留学回国人员科研启动基金(No.教外司留[2008]890号);云南省科技计划项目(2007B035M)资助

摘　　要：类固醇类激素以其独特的生理生化效应而备受关注,气相色谱-质谱联用技术已经成为类固醇类激素分析检测的重要手段。由于类固醇类激素含有羟基、酮基等极性官能团,GC-MS分析时检测灵敏度不高,色谱分离峰形差,需通过衍生化技术将其转化成极性较低、易挥发、热稳定性高的化合物,以改善色谱分离峰形、提高检测灵敏度,使之适用于GC-MS痕量分析。目前,类固醇类激素羟基等含有活性氢原子官能团的衍生化技术发展较为成熟,但酮基衍生化仍缺乏系统研究。本文对类固醇类激素酮基衍生化技术研究进展进行了综述,主要介绍了烯醇化-硅烷化、肟化-硅烷化等酮基衍生化技术的反应原理、应用现状,并对目前存在的问题及进一步的研究方向进行了讨论和展望,为类固醇类激素酮基衍生化技术的研究提供参考。Steroid hormones have poor detection sensitivity and chromatography peaks because of their polar functional groups such as keto groups and hydroxyl groups. So these groups need to transform into derivatives which have lower polar, more volatile and higher thermal stability to suitable for the trace GC-MS analysis. The derivatization technology of hydroxyl groups and other functions which containing active hydrogen atom has been greatly developed, but it is still necessary to do systematic research on the keto group derivatization. In this article, keto groups derivatization of steroid hormones were reviewed, including reaction principle, application status of enolisation-silylation, oximation-silylation derivatization technology etc. The problems and research direction were also discussed.

关 键 词：类固醇类激素 酮基 衍生化 气相色谱-质谱联用 

分 类 号：TQ467[化学工程—制药化工]