迟发性阿尔茨海默病的肿瘤坏死因子-αG-308A基因多态性研究  

TNF-α G-308A gene polymorphism of late-onset Alzheimer disease

作　　者：张许来[1] 张晓莉[1] 陶领知[1] 孔晓明[1] 张丽[1] 李泽爱[1] 张胜权[2] 

机构地区：[1]合肥市精神病医院,230022安徽合肥 [2]安徽医科大学分子生物学教研室

出　　处：《临床心身疾病杂志》2010年第6期485-486,497,共3页Journal of Clinical Psychosomatic Diseases

基　　金：基金项目：合肥市科技局2006年重点科研资助项目（编号2006-27）

摘　　要：目的 探讨迟发性阿尔茨海默病的肿瘤坏死因子-α G-308A基因多态性.方法 将57例迟发性阿尔茨海默病患者设为研究组,抽取52例健康志愿者设为对照组,采用聚合酶链反应、限制性片段长度多态性方法检测两组肿瘤坏死因子-α G-308A多态性. 结果 两组脂蛋白E-4等位基因分布显著不同（χ2=19.39,P〈0.01）,脂蛋白E-4等位基因携带者罹患迟发性阿尔茨海默病风险很高（OR=2.49,95%CI 1.53-4.07,P〈0.01）;肿瘤坏死因子-αAA基因与脂蛋白E-4等位基因的交互作用致迟发性阿尔茨海默病风险增高（χ2=6.34,P〈0.05）;研究组脂蛋白E-4等位基因非携带者中,AA、AG基因型频率,A-等位基因频率显著高于对照组（χ2=5.26、4.58,P〈0.05）. 结论 肿瘤坏死因子-α G-308A基因多态性可能增加迟发性阿尔茨海默病发病风险,同时这种多态性与脂蛋白E-4等位基因状态有关.Objective To explore the TNF-α G-308A gene polymorphism ot late-onset Alzheimer disease （LAD）. Methods Fifty-seven LAD patients were assigned to research group and 52 healthy volunteers to control group. The TNF-α G-388A gene polymorphism of both groups was detected with the Polymerase Chain Reaction （PCR） and the Restriction Fragment Length Polymorphism （RFLP）. Results Distribu- tions of apolipoprotein E-4 alleles of the two groups were notably different （χ2= 19.39 ,P〈0.01）, the risk suffering from LAD was very high in apolipoprotein E allele carriers （OR=2.49,95 %CI 1.53-4.07,P〈 0.01） ; the interaction of TNF-α AA and apolipoprotein E allele produced increased risk for LAD （χ2 = 6.34,P〈0.05）; AA, AG genotype and A-allele frequency were significantly higher in the patients with- out apolipoprotein E allele of the research than in the volunteers （χ2= 5.26,4.58, P〈0.05）. Conclusion TNF-α G-308A gene polymorphism possibly increase the onset risk for LAD and the polymorphism is related to the status of apolipoprotein E allele at the same time.

关 键 词：阿尔茨海默病 载脂蛋白E-4等位基因 肿瘤坏死因子-Α 多态性 

分 类 号：R749.16[医药卫生—神经病学与精神病学]