克罗卡林对急性脑缺血再灌注大鼠的脑保护作用  

Protective effect of Cromakalin on rats following acute cerebral ischemia-reperfusion

作　　者：冯涛[1] 娄季宇[1] 白宏英[1] 杨霄鹏[1] 曾志磊[1] 王金兰[1] 

机构地区：[1]郑州大学第二附属医院神经内科,郑州450014

出　　处：《中国实用神经疾病杂志》2010年第24期11-13,共3页Chinese Journal of Practical Nervous Diseases

摘　　要：目的探讨急性脑缺血再灌注后大鼠脑内小胶质细胞的活化及K-ATP通道开放剂克罗卡林(Cromakalin)的脑保护作用。方法将72只SD大鼠,随机分为3组:A组为假手术组,B组为单纯缺血再灌注组,C组为克罗卡林干预组。应用改良Zea Longa线栓法制备大鼠大脑中动脉缺血再灌注(MCAO)模型,免疫组化染色检测OX42(小胶质细胞的标志)和诱导型一氧化氮合酶(iNOS)表达情况;应用特异性尼氏染色进行海马CA1区神经元计数。结果 A组各时间点海马CA1区OX42,iNOS均有少量表达,B组OX42,iNOS随时间推移表达增多,72h达高峰,7d减少,与A组相比两者表达明显增多,差异有统计学意义(P<0.01)。C组各时间点OX42、iNOS与B组相比表达明显减少,差异有统计学意义(P<0.01)。结论 Cromakalin能明显抑制急性脑缺血再灌注后大鼠脑内OX42增殖,减少iNOS的表达,降低海马神经元的死亡程度,具有脑保护作用。Objective To investigate the activated microglia cells and the protective effect of K-ATP channel opener Cromakalin on rats following acute cerebral ischemia-reperfusion. Methods Seventy-two SD rats were randomly divided into three groups including sham-operated group （Group A）, ischemia-reperfusion group （Group B）, Cromakalin intervention group （Group C）. MCAO model was established according to Zea Longa methods. The expression of OX42 and iNOS were examined by immunohistochemical staining. The numbers of neurons were examined by Nissl＇s staining. Results In CA1 of hippocampus, there were a few expressions of OX42 and iNOS in group A. In group B, the expressions of OX42 and iNOS increased over time, reducing at 7th day. Compared with group A, there was a significant difference （P〈0.01）. Compared with B group, the expressions of OX42 and iNOS in group C reduced, there was a significant difference （P〈0.01）. Conclusion Cromakalin can significantly reduce the expressions of OX42, iNOS and the death of neurons in the CA1 of hippocampus ; it has the protective effect with brain.

关 键 词：脑缺血再灌注 小胶质细胞 克罗卡林 脑保护 

分 类 号：R-33[医药卫生]