不同贮藏温度对银中杨花粉寿命的影响  

Live Span of Pollen of Populus alba×P. berolinensis in Different Storage Temperature

作　　者：程广有[1] 唐晓杰[1] 孟范例[1] 魏春媛[1] 

机构地区：[1]北华大学林学院,吉林吉林132013

出　　处：《北方园艺》2010年第23期105-107,共3页Northern Horticulture

摘　　要：设定了-90、-30、-15、5℃和室温5个水平贮藏银中杨花粉,用氯化2,3,5-三苯基四唑(TTC)染色法测定花粉生活力。结果表明:银中杨单株间花粉寿命差异显著;银中杨花粉生活力因贮藏温度不同而存在显著差异,温度越低花粉寿命越长。室温条件下,银中杨花粉1周后即丧失活力;在5℃贮藏10 d时,有生活力花粉为22.98%,丧失活力;15 d后在-15℃和-30℃条件下贮藏,15 d后有生活力花粉分别为31.98%和38.56%;在-90℃条件下贮藏15 d后,有生活力花粉为82.7%,30 d时有活力的花粉为50.68%。Five temperature levels of storage of poplar pollen were set. Pollen live of Populus alba × P. berolinensis was studied at different temperature, they were - 90, - 30, - 15,5℃ and nature temperature, with chlorinated 2, 3, 5- Triphenyl tetrazole （TTC）. The results showed that there was significant variation of longevity of poplar pollen among plants. There was significant variation of longevity of poplar pollen among different storage temperature, too. The lowwer the temperature of storage, the longger life expectancy. Poplar pollen can save one week at nature temperature. There were life pollen 22. 98% aftter 10 days at 5℃. There were life pollen 31. 98% and 38. 56% at-15℃ and -30℃. There were life pollen 82. 7% and 50. 68% after 15 d and 30 d at -90℃ separately.

关 键 词：银中杨 花粉生活力 贮藏温度 

分 类 号：S792.119[农业科学—林木遗传育种]