人参皂甙Rg1与1,6-二磷酸果糖配伍抗疲劳作用的研究  

Compatible Effect of Ginsenoside Rg1 and 1,6-Fructose Diphosphate on Anti-fatigue

作　　者：陈林军[1,2] 王莹[2] 蔡东联[2] 林宁[2] 朱炜[2] 曲丹[2] 郑慧[2] 

机构地区：[1]上海市职工医学院公共卫生系,上海200234 [2]第二军医大学附属长海医院临床营养科,上海200433

出　　处：《氨基酸和生物资源》2010年第4期58-62,共5页Amino Acids & Biotic Resources

基　　金：上海市体育局科研基金项目(04JT017)

摘　　要：观察人参皂甙Rg1与1,6-二磷酸果糖配伍的抗疲劳效果,寻找最佳配伍剂量。根据雄性清洁级昆明种小鼠按体重随机分为安静对照组、运动对照组、人参皂甙Rg1对照组及4个配伍组。通过析因实验设计分析人参皂甙Rg1与1,6-二磷酸果糖提高运动耐力的效果及两者的交互作用。结果不同剂量人参皂甙Rg1对小鼠力竭游泳时间影响有显著差异(P<0.05)。各配伍组力竭游泳时间均有显著延长,C组延长最显著;与人参皂甙Rg1对照组相比,各配伍组力竭游泳时间均显著降低(P<0.01)。MDA含量A组显著低于运动对照组和D组,与安静对照组相比,各组均升高。SOD/MDA比值A、C组显著高于运动对照组,C组显著高于人参皂甙Rg1对照组,与安静对照组相比,各组均有下降趋势,D组下降最显著(P<0.01)。乳酸脱氢酶C、D组显著低于运动对照组和人参皂甙Rg1对照组(P<0.05)。与运动对照组及人参皂甙Rg1对照组相比,配伍在一定程度上减少心肌、骨骼肌细胞线粒体和其他细胞超微结构损伤。结论:与FDP配伍未能延长小鼠力竭游泳时间,配伍可减轻耐力运动对小鼠心肌和骨骼肌的细胞损害,一定程度上保护线粒体的呼吸功能,缓解细胞缺氧损伤。To observe the compatible effect of ginsenoside Rg1 and 1,6-fructose diphosphate on anti-fatigue,and to determine the optimal dose for compatibility,Male Kunming mice were randomized according to their body weight into 7 groups in total.3 control groups（namely the quiet,exercise,and ginsenoside Rg1 control groups） and 4 treatment ones with different dosage of ginsenoside Rg1 and 1,6-fructose diphosphate（namely Group A,Group B,Group C and Group D）.The effect and interaction of ginsenoside Rg1 and 1,6-fructose diphosphate on enhancing exercise tolerance were analyzed via factorial experiment design.Factorial analysis suggested significant difference in the duration of give-out swimming of mice between different dosage of ginsenoside Rg1 groups（P0.05）.The duration of give-out swimming in all treatment groups witnessed significant extension,and the greatest extension was seen in group C.Compared to ginsenoside Rg1 control group,however,the duration in all treatment groups significantly lowered（P0.01）.MDA content of group A was significantly lower than that of Group D and the exercise control group.The MDA content of all groups elevated compared to the quiet control group.The ratio between SOD and MDA in group A and C was significantly higher than the exercise control group,while the ratio in group C was significantly higher than the ginsenoside Rg1 control group.All groups witnessed a decline in SOD：MDA ratio compared to the quiet control group,and group D enjoyed the greatest significance（P0.01）.LDH in group C and D was significantly lower than in the exercise and ginsenoside Rg1 control group（P0.05）.As a result,compared to the exercise and ginsenoside Rg1 control group,compatibility reduced,to some extent,the impairment of myocardial and skeletal muscle cells,mitochondrion,and other ultrastructure of cells.Compatibility with 1,6-fructose diphosphate did not extend the duration of give-out swimming of mice,however,it could reduce the impairment of myocardial and skeletal muscle cells,protec

关 键 词：人参皂甙RG1 抗疲劳 1 6-二磷酸果糖 析因分析 小鼠 

分 类 号：R15[医药卫生—营养与食品卫生学]