组织器官特异性基因差异表达在毒理学中的应用  

APPLICATION OF TISSUES AND ORGANS-SPECIFIC GENE DIFFERENTIAL EXPRESSION IN TOXICOLOGY

作　　者：黎运西[1,2] 朱家勇[1,2] 金小宝[1,2] 曾爱华[3] 褚夫江[1,2] 

机构地区：[1]广东药学院基础学院,广州510006 [2]广东省生物活性药物研究重点实验室 [3]广东药学院药科学院

出　　处：《现代预防医学》2010年第23期4526-4528,共3页Modern Preventive Medicine

基　　金：广东省教育部产学研结合项目(2008B090500191);广东省产业技术研究与开发项目(2008B080703065)资助

摘　　要：传统的毒理学检验方法研究时程较长,敏感性低,且有些毒性效应未能被检出。近年来有不少文献报道用基因组学和蛋白组学方法进行药物安全性评价,本文就组织器官特异性基因转录和翻译的表达差异,在药物毒性试验中的应用原理,如何应用mRNA转录和蛋白的翻译表达变化来研究器官特异毒性作用,如何运用毒性基因表达探讨动物机体内肝肾等多种器官特异毒性作用的机制等最新应用情况进行综述,为其更好地应用于新药开发中的毒性试验和药物的临床试验提供参考。The traditional methods of toxicological assessment are time consuming and insensitive,and some toxic effects can not be detected.For the past years,the technologies used to measure expression at the transcript and protein levels for safety assessment of new drugs are reported by considerable papers.This paper reviews the latest research about the principle of new drugs＇ safety evaluation by measuring the specific gene expression of animal tissue or organ in the toxicity tests in vivo,the methods and the mechanism of studying the new drugs＇ toxicity by measuring mRNA and protein levels to elevate the sensitivity of new drugs＇ safety assessment and improve concordance between animal toxicity test and clinical trial.

关 键 词：基因表达 毒性试验 临床前研究 

分 类 号：R394[医药卫生—医学遗传学]