甲状腺癌原位细胞凋亡检测与细胞核因子-kB的表达及意义  

Expression and significance of nuclear factor-kB and apoptosis index in thyroid carcinoma

作　　者：杨菊[1] 邓涛[1] 黄燕凤[1] 步鸣[2] 钱立勇[1] 李春生[1] 王兆宇[1] 王宏强[1] 

机构地区：[1]浙江省舟山医院,浙江舟山316004 [2]苏州大学医学部,江苏苏州215123

出　　处：《现代实用医学》2010年第10期1101-1103,1120,共4页Modern Practical Medicine

摘　　要：目的研究原位细胞凋亡指数(AI)及细胞核因子-B(NF-B)在原发性甲状腺癌中的表达及其与病变的关系。方法采用原位细胞凋亡检测(TUNEL)试剂盒及免疫组化方法分别检测原发性甲状腺癌(75例)和正常甲状腺组织(10例)的AI及NF-B表达情况。结果甲状腺癌组织AI值明显高于正常甲状腺组织(P<0.05),不同病理分型的甲状腺癌组织AI值阳性情况表达差异无统计学意义(P>0.05);甲状腺癌组织中NF-B的表达明显高于正常甲状腺组织(P<0.05),不同病理分型的甲状腺癌组织NF-B表达差异无统计学意义(P>0.05)。甲状腺癌组织AI值阳性情况表达与NF-B的表达呈正相关(r=0.506,P<0.01)。结论在甲状腺癌中NF-B的高表达影响了甲状腺癌组织中的细胞凋亡。Objective Tostudytheexpressionandsignificanceofnuclearfactor-B（NF-kB）andapoptosisindexinthy-roid carcinoma.Methods 75 patients with thyroid carcinoma and 10 normal thyroid were studied for detection of ap-optotic index by TUNEL method,and the expression of NF-kB by immunohistochemistry.Results The mean apop-tosis index in thyroid carcinoma and normal thyroid group were 20.08±4.39 and 10.70±2.66,respectively（P〉0.05）.There was no significant association between apoptosis index and the histological type.The expression of NF-kB in thy-roid cancer was obviously higher than that in normal thyroid tissue（P〉0.05）.There were no significant difference among different pathological type of cancer（P〉0.05）.There was a significantly positive correlation between apoptosis index and the expression of NF-kB in thyroid carcinoma（r=0.506,P〉0.01）.Conclusions High expression of NF-kB in thyroid cancer which affects the apoptosis index of the thyroid carcinoma.

关 键 词：甲状腺癌 免疫组化 凋亡指数 细胞核因子-B 

分 类 号：R736.5[医药卫生—肿瘤]