变异型酪氨酸酶对治疗黑色素代谢异常疾病的研究  

Tyrosinase treatment of variant pigment metabolic disease research

作　　者：陈菁[1] 刘树滔[2] 郑淑霞 邓文汉[2] 饶平凡[2] 

机构地区：[1]福州大学生物科学与工程学院实验中心,福州350108 [2]福州大学生物工程研究所,福州350002 [3]福建省中医药研究所,福州350000

出　　处：《海峡药学》2010年第11期242-245,共4页Strait Pharmaceutical Journal

基　　金：福建省自然科学基金重大科技项目(2001F009);福州大学发展基金资助项目(2002-XY-11XJY-0241XKJ(QD)-0132)

摘　　要：本文在野生型酪氨酸酶(TYR)的基础上,运用分子生物学手段成功构建两种变异型表达质粒pET-Tat-TYR(含双精氨酸分泌信号肽变异型)、pGEX-TYR(缺失C端跨膜区的GST融合变异型),表达了两种变异型酪氨酸酶,并分别对产物进行了分析。该研究结果拓宽了蛋白药物在递送方面的应用范围,为酪氨酸酶在治疗色素代谢异常疾病方面开辟了新的视野,并为酪氨酸酶的有效应用提供理论指导。This paper was on the basis of the wild-type tyrosinase（TYR）.Two variant expression plasmid pET-Tat-TYR（including double-arginine signal peptide variant）and pGEX-TYR（missing C terminal transmembrane District of GST fusion variant）were constructed successfully by the use of molecular biology methods.The expression of two variants of tyrosinase,and the products were analyzed separately.The results broaden the delivery of protein drugs in the application scope,for the tyrosinase treatment of metabolic disorders pigments open up new horizons for the effective application of tyrosinase to provide theoretical guidance.

关 键 词：变异型酪氨酸酶 表达 黑色素代谢异常 

分 类 号：Q786[生物学—分子生物学]