麻疯树种子中抗氧化活性成分的研究  

Chemical constituents of anti-oxidative activities from seeds of Jatropha curcas

作　　者：李玲[1,2] 李晓帆[2] 吴慧星[1] 李荣[1] 王乃利[1,2] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]深圳清华大学研究院深圳市创新中药及天然药物研究重点实验室,广东深圳518057

出　　处：《中草药》2010年第12期1932-1936,共5页Chinese Traditional and Herbal Drugs

摘　　要：目的研究麻疯树Jatropha curcas种子中具有抗氧化活性的有效成分。方法使用DPPH活性测试方法进行抗氧化活性的筛选和追踪,采用活性追踪性分离方法,使用硅胶、ODS和凝胶柱色谱等现代分离手段分离得到麻疯树种子中具有抗氧化活性的有效成分。综合应用多种光谱学方法(UV、IR、ESI-MS、HR-ESI-MS、1H-NMR、13C-NMR、2D-NMR)对所得化合物进行解析并鉴定其结构。最后使用DPPH法测定并评价单体化合物的抗氧化活性。结果麻疯树种子提取物的正丁醇萃取层具有显著的抗氧化活性,通过对该萃取部分的成分研究共分离得到6个化合物,经结构解析,分离得到的6个化合物分别鉴定为麻疯果素A(3,4,4′5′-四羟基-3′-甲氧基-双环氧木脂素,3,4,4′,5′-tetrahydroxyl-3′-methoxylbisepoxylignan,1)、(±)-3,3′-bisdemethylpinoresinol(2)、7-表-芝麻素-二儿茶酚(3)、异巴西油大戟素(isoprincepin,4)、β-谷甾醇(5)与β-胡萝卜苷(6)。结论活性测定结果表明,化合物3、4具有较强活性,化合物1、2具有中等强度活性。化合物1为新化合物,命名为麻疯果素A(jatropha-sin A);化合物2、3、4为首次从麻疯树属中分离得到。Objective To study the chemical constituents with anti-oxidative activities from the seeds of Jatropha curcas.Methods DPPH Radical scavenging assay was used for screening the active fractions.Using activity-guided-isolation,the active fractions were separated by column chromatography with silica gel,ODS,and Sephadex LH-20.The isolated compounds were then identified by spectral analyses（UV,IR,ESI-MS,HR-ESI-MS,1H-NMR,13C-NMR,and 2D-NMR）.Anti-oxidative activities of all the isolated compounds were determined finally.Results The n-butanol layer of ethanol extract from the seeds of J.curcas showed stronger activity and was studied further.Six compounds were isolated from this active layer and their structures were identified as jatrophasin A（3,4,4′,5′-tetrahydroxyl-5′-methoxyl-bisepoxylignan,1）,（±）-3,3′-bisdemethylpinoresinol（2）,7-epi-sesamin-dicatechol（3）,isoprincepin（4）,β-sitosterol（5）,and β-daucosterol（6）.Conclusion Compounds 3 and 4 show the strong,1 and 2 show the medium anti-oxidative activities.Compound 1 is a new compound,named jatrophasin A,compund 2 is a new natural compound and 3,4 are reported for the first isolation from the plants of Jatropha L.

关 键 词：麻疯树种子 抗氧化活性 麻疯果素A 麻疯果素B 

分 类 号：R284.1[医药卫生—中药学]