VEGF p53和p21 ras在原发性肝细胞癌中的表达及其临床病理意义  

CLINICAL PATHOLOGICAL SIGNIFICANCE OF VEGF,p53 AND p21RAS PROTEIN EXPRESSION IN HEPATOCELLULAR CARCINOMA

作　　者：纪子钊[1,2] 王宝胜[1,2] 张铭链 余云[1,2] 王之章 刘峰[1,2] 

机构地区：[1]中国医科大学第二临床学院普外科 [2]鞍山钢铁公司铁东医院普外科 [3]中国医科大学第二临床学院病理科

出　　处：《中国现代医学杂志》1999年第4期6-8,共3页China Journal of Modern Medicine

摘　　要：目的：研究ＶＥＧＦ，ｐ５３和ｐ２１ｒａｓ在原发性肝细胞癌（ＨＣＣ）的表达和三者间的相关性及其与ＨＣＣ病理分级，转移行为的关系。方法：应用免疫组织化学Ｓ－Ｐ法对３４例ＨＣＣ的癌组织中的ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３的表达作了研究。结果：ＶＥＧＦ，ｐ２１ｒａｓ和ｐ５３三者在肝癌和癌旁组织的阳性率均有非常显著的差异（Ｐ＜０．０１），ＶＥＧＦ的表达与ｐ２１ｒａｓ和ｐ５３均呈显著的正相关（Ｐ＜０．０１）。三者间的协同表达亦与ＨＣＣ的病理分级和转移行为有关，而与肿瘤大小，ＡＦＰ水平，ＨＢｓＡｇ阳性无关。结论：ＶＥＧＦ可能在ＨＣＣ的形成，发展和转移中与ｐ５３和ｒａｓ基因协同作用，三者的协同作用可能与ＨＣＣ的病理分级和转移行为有关。Objective:To study the correlation between expressions of VEGF,p53,p21ras and cell differentiation and metastasis in hepatocellular carcinoma(HCC).Methods:34 cases of HCC were selected for this study.Expressions of VEGF,Mutant p53(M53)and p21ras pro-tein were synchronously detected and compared by immunohistochemical S-P method.Results:The positive rates of VEGF,Mp53,p21ras in HCC were very significantly higher than that of PT(<0.01).VEGF and Mp53 protein were positive simultaneously in 17(50%)in HCC which had positive correlation(P<0.01);VEGF and p21ras protein were positive simulaneously in 22(64.7%)which had positive correlation too(P<0.01);A signifficant correlation was found between the coexpression was associated with the degree of histologic differentiation and metastasis behavior in HCC,but not with tumor size,AFP levels and HBsAg infection.Conclusion:The results showed the expression of VEGF was related with p53 and p21ras.The coexpression of them may be useful marks for evaluating the malignant degree,the metastasis and invasive ability of HCC.

关 键 词：肝细胞癌 血管内皮 生长因子 P53基因 RAS基因 

分 类 号：R735.702[医药卫生—肿瘤]