血小板活化因子对神经细胞内游离钙离子作用的研究  

The effect of platelet activating factors on cytosolic free calcium of cultured neurons from cerebral cortex in rats

作　　者：王伟[1,2] 徐建华 

机构地区：[1]同济医科大学同济医院神经内科 [2]华中理工大学生物物理与生物化学研究所

出　　处：《中华神经科杂志》1999年第2期83-85,共3页Chinese Journal of Neurology

基　　金：国家自然科学基金

摘　　要：目的观察脑损害时病理活性介质血小板活化因子（ｐｌａｔｅｌｅｔａｃｔｉｖａｔｉｎｇｆａｃｔｏｒ，ＰＡＦ）及其拮抗剂对神经细胞内游离钙离子（［Ｃａ２＋］ｉ）浓度的影响，探讨ＰＡＦ致使神经细胞内［Ｃａ２＋］ｉ超载的途径及其作用机制。方法采用ＡＲＣＭＭＩＣ阳离子检测系统，观察不同浓度的ＰＡＦ以及其特异性受体拮抗剂ＢＮ５２０２１和非特异性拮抗剂ＭＫ８０１对体外培养１４天的大鼠皮层神经元［Ｃａ２＋］ｉ浓度的影响。结果５×１０－６ｍｏｌ／Ｌ的ＰＡＦ可使培养神经细胞［Ｃａ２＋］ｉ浓度明显增高，与对照组比较差异有显著意义（Ｐ＜０．０１）；ＢＮ５２０２１可明显阻止ＰＡＦ所致的神经细胞内［Ｃａ２＋］ｉ超载，在此基础上加用ＭＫ８０１可使神经细胞内［Ｃａ２＋］ｉ水平下降的更为明显。结论ＰＡＦ可通过多种渠道致使神经细胞内钙离子超载。Objectives To determine the effect of platelet activating factor (PAF) on cytosolic free calcium ([Ca 2+ ]i) of cultured neurons from cerebral cortex in rats and their pathepenetic effect on neuronal calcium overload and ceubal brain. Methods The primary neuron cultured for 14 days was obtained and the effect of PAF in different concentrations on [Ca 2+ ]i was observed by the AR CM MIC cation measurement system .The route of PAF increase [Ca 2+ ]i was observed with BN52021 and MK 801. Results The [Ca 2+ ]i was elevated by PAF in the concentration of 5×10 -6 mol/L ( P <0.01), but the effect was blocked by the specific PAF receptor antagonist BN52021. The effect of BN52021 was strengthened by MK 801. Conclusion [Ca 2+ ]i of the cultured neurons is elevated by PAF. PAF receptor antagonist BN52021 and MK 801 have a potent resistant effect on PAF.

关 键 词：血小板活化因子 钙 神经元 脑损害 

分 类 号：R742.02[医药卫生—神经病学与精神病学]