β_1整合素表达下调对人结肠癌细胞增殖和化疗敏感性影响  

EFFECT OF DOWN-REGULATION OF INTEGRIN-β_1 EXPRESSION ON CELL PROLIFERATION AND CHEMOSENSITIVITY OF HUMAN COLON CANCER HT-29 CELLS

作　　者：张建立[1,2] 高军[2] 谭晓杰[2] 王敏[1] 秦仁义[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院普外科,湖北武汉430030 [2]青岛大学医学院附属医院普外科

出　　处：《齐鲁医学杂志》2010年第6期471-472,475,共3页Medical Journal of Qilu

基　　金：山东省自然科学基金资助项目(Y2007C030)

摘　　要：目的观察β1整合素表达下调对人结肠癌HT-29细胞增殖和化疗敏感性的影响。方法采用反义寡核苷酸(ASODN)技术转染HT-29细胞,逆转录-聚合酶链反应(RT-PCR)检测β1整合素表达下调情况。MTT法检测HT-29细胞相对存活率。给予梯度浓度氟尿嘧啶(5-FU),计算IC50。结果实验组β1整合素条带吸光度积分(IAD)值/β-actin条带IAD值的比值及HT-29细胞的存活率与对照组相比显著降低(F=1630.08,q=72.40,P<0.01;t=4.37,P<0.05)。5-FU+ASODN组HT-29细胞对5-FU的IC50与5-FU组比较显著下降(t′=37.71,P<0.05)。结论β1整合素表达下调可抑制结肠癌细胞增殖,增强结肠癌细胞对化疗药物敏感性。Objective To investigate the effect of down-regulation of integrin-β1 expression on cell proliferation and chemosensitivity of human colon cancer cell line HT-29. Methods HT 29 cells were transfected with antisense oligonucleotide technology, the down-regulation of integrin-β1 mRNA expression was detected by RT-PCR. The relative survival rate of HT-29 cells was determined by MTT method. Gradient concentrations of 5 fluorouracil （5-FU） were given, and IC50 calculated. Results In comparison with the control group, the expression of integrin-[31 mRNA of HT-29 cells in the experimental group was markedly down-regulated （F=1630.08,q=72.40,P〈0. 01）, and the survival rate of HT 29 cells was significantly reduced （t=4.37,P〈0.05）, and the IC50 remarkably decreased （t＇=37.71 ,P〈0.05）. Conclusion Down-regulation of integrin-β1 can effectively inhibit cell proliferation and enhance the sensitivity of human colon cancer cell to chemotherapeutics.

关 键 词：结肠肿瘤 抗原 CD29 细胞增殖 药物筛选试验 抗肿瘤 

分 类 号：R735.35[医药卫生—肿瘤]