Nectin-4与卵巢癌的关系及其意义  

Relationship between Nectin-4 and ovarian cancer and the significance

作　　者：张萍[1] 柴守辉[2] 高媛[1] 

机构地区：[1]山东省青岛市市立医院妇科,266011 [2]滕州市中心人民医院妇科

出　　处：《中国妇幼保健》2010年第34期5095-5098,共4页Maternal and Child Health Care of China

基　　金：青岛市科技发展指导性计划项目(09KZJ-21)

摘　　要：目的:检测Nectin-4在卵巢癌中的表达情况,分析其与卵巢癌临床病理参数之间的关系。方法:RT-PCR法检测30例卵巢癌、25例卵巢良性肿瘤和30例正常卵巢组织Nectin-4 mRNA表达;Western blotting法检测Nectin-4蛋白表达。结果:①Nectin-4 mRNA表达为卵巢癌组0.71±0.12,卵巢良性肿瘤组0.32±0.09、正常卵巢组0.30±0.09,卵巢癌组显著高于后两组,差异有统计学意义(P<0.01)。卵巢癌组织中,不同年龄、病理类型间比较,差异均无统计学意义(P>0.05);不同病理分化程度、手术病理分期、有无淋巴结转移比较,差异均有统计学意义(P<0.05)。②Nectin-4蛋白表达为卵巢癌组0.71±0.06、卵巢良性肿瘤组0.35±0.07、正常卵巢组0.32±0.07,卵巢癌组织明显高于卵巢良性肿瘤和正常卵巢组织,差异有统计学意义(P<0.01)。结论:卵巢癌存在Nectin-4过度表达,与其病理分化程度、手术病理分期及淋巴结转移有密切关系,提示Nectin-4参与调控卵巢癌发生及发展过程。Objective：To detect the expression of Nectin-4 in ovarian cancer,analyze the relationship between Nectin-4 expression and clinicopathological parameters of ovarian cancer.Methods：RT-PCR method was used to detect the expression levels of Nectin-4 mRNA in 30 cases with ovarian cancer,25 cases with benign ovarian tumor and 30 cases with normal ovary;the expression levels of Nectin-4 protein were detected by Western blotting.Results：The expression level of Nectin-4 mRNA in ovarian cancer group was（0.71±0.12）,which was significantly higher than those in benign ovarian tumor group（0.32±0.09） and normal ovary group（0.30±0.09）（P〈0.01）;in ovarian cancer group,there was no significant difference among different ages and pathological types（P〉0.05）;there was significant difference among different pathological differentiation degrees,surgical pathological stages and lymphatic metastasis or not（P〈0.05）.The expression level of Nectin-4 protein in ovarian cancer group was（0.71±0.06）,which was significantly higher than those in benign ovarian tumor group（0.35±0.07） and normal ovary group（0.32±0.07）（P〈0.01）.Conclusion：The cases with ovarian cancer has over-expression of Nectin-4,which is related to pathological differentiation degrees,surgical pathological stages and lymphatic metastasis,so it is indicated that Nectin-4 is involved in the occurrence and development of ovarian cancer.

关 键 词：Nectin-4 卵巢癌 细胞黏附 

分 类 号：R737.31[医药卫生—肿瘤]