MnO2纳米溶胶-抗坏血酸化学发光反应的研究  

Investigation on the chemiluminescence reaction of nano-colloidal MnO_2 and ascorbic acid

作　　者：王虹[1] 杜建修[1] 

机构地区：[1]陕西省生命分析化学重点实验室,陕西师范大学化学与材料科学学院,西安710062

出　　处：《分析试验室》2010年第12期43-46,共4页Chinese Journal of Analysis Laboratory

基　　金：中央高校基本科研业务费专项资金(GK200902012);陕西师范大学优秀科技预研项目(200902016)资助

摘　　要：在中性条件下利用Na2S2O3还原KMnO4制备出平均粒径约40 nmMnO2溶胶。研究发现,该溶胶在酸性条件下可氧化抗坏血酸产生化学发光。基于此,结合流动注射技术,对影响化学发光反应的各种因素进行了考察,建立了测定抗坏血酸的流动注射化学发光新方法。该方法测定抗坏血酸的线性范围为2.0×10-6~1.0×10-4g/mL,检出限为4×10-7g/mL,相对标准偏差为2.1%（1.0×10-5g/mL抗坏血酸溶液,n=11）。该方法已用于片剂中抗坏血酸的测定,结果与药典方法一致。Nano-colloidal MnO2 with an average diameter of 40nm was prepared by reducing KMnO4 with Na2S2O3 in neutral solution.A new flow injection chemiluminecence method was developed for the determination of ascorbic acid,based on the chemiluminescence from the oxidation of ascorbic acid by nano-colloidal MnO2 under acidic condition.The experimental conditions were optimized using flow-injection method.Under the selected conditions,the linear range for ascorbic acid was 2.0×10-6~1.0×10-4 g/mL and the detection limit was 4×10-7g/mL ascorbic acid.The relative standard deviation was 2.1% for the 11 repeated determinations of 1.0×10-5 g/mL ascorbic acid solution.The method has been applied to the determination of ascorbic acid in tablets and the results were in good agreement with those obtained by official method.

关 键 词：MnO2纳米溶胶 化学发光 流动注射 抗坏血酸 

分 类 号：O657.3[理学—分析化学]