化学计量学-同步荧光光谱法同时测定速灭威、残杀威和呋喃丹  

Simultaneous synchronous fluorimetric determination of metolcarb,propoxur and carbofuran by chemometrics methods

作　　者：李蔚博[1] 张国文[1] 赵楠[1] 胡兴[1] 

机构地区：[1]南昌大学食品科学与技术国家重点实验室,南昌330047

出　　处：《分析试验室》2010年第12期59-62,共4页Chinese Journal of Analysis Laboratory

基　　金：江西省科技支撑计划项目(2009BNA09000);国家质检总局科技项目(2009IK141);南昌大学食品科学与技术国家重点实验室目标导向项目(SKLF-MB-200807)资助

摘　　要：在pH 4.0的B-R缓冲溶液中,速灭威、残杀威和呋喃丹能够产生内源荧光,但光谱严重重叠。当波长差Δλ=30 nm时,加入一定量的β-环糊精,3种农药的荧光强度均有不同程度的加强,且荧光强度与农药的浓度呈良好的线性关系,速灭威、残杀威和呋喃丹单组分的测量线性范围分别为0.2~2.0、0.02~0.38和0.04~0.56μg/mL;检出限分别为0.083、0.015和0.020μg/mL。应用多种化学计量学方法,如经典最小二乘（CLS）、主成分回归（PCR）和偏最小二乘（PLS）对同步荧光光谱严重重叠的3种农药混合体系合成样进行光谱解析并比较其分析能力。结果表明,PLS预报各组分的结果较好,采用该模型分析了一些实际食品样品,获得满意的结果。Three carbamate pesticides of metolcarb,propoxur and carbofuran can produce intrinsic fluorescence in a B-R buffer medium of pH 4.0,but their fluorescence spectra overlapped seriously.The fluorescence intensity of the pesticides can be enhanced separately in the presence of β-cyclodextrin（β-CD） with Δλ=30 nm,and the fluorescence intensity is proportional to the concentration of the pesticides.The linear ranges for metolcarb,propoxur and carbofuran are 0.2~2.0,0.02~0.38 and 0.04~0.56 μg/mL,respectively,and the limits of detection are 0.083 μg/mL for metolcarb,0.015 μg/mL for propoxur and 0.02 μg/mL for carbofuran.The different chemometrics methods as the classical least squares（CLS）,principal components regression（PCR） and partial least squares（PLS） were tested by the use of a validation data set constructed from synthetic solutions of these three compounds.The analytical performance of these chemometrics models was compared.It was found that the PLS gave the better results than other chemometrics models,which was then applied to the simultaneously determination of the three pesticides in real food samples with satisfactory results.

关 键 词：化学计量学 同步荧光光谱法 氨基甲酸酯农药 同时测定 

分 类 号：O657.3[理学—分析化学]