石油污染土壤中细菌群落结构特征  

CHARACTERISTICS OF BACTERIAL COMMUNITY IN LONG-TERM OIL POLLUTED SOIL

作　　者：万春黎[1] 杨雪[1] 杜茂安[1] 于春光[1] 李观元[1] 

机构地区：[1]哈尔滨工业大学市政环境工程学院,黑龙江哈尔滨150090

出　　处：《石油学报（石油加工）》2010年第6期928-933,共6页Acta Petrolei Sinica(Petroleum Processing Section)

基　　金：国家重大基础研究发展计划"863"项目(2007AA061200);黑龙江省教育厅科学技术研究项目(11544038)资助

摘　　要：采用聚合酶链式反应(PCR)技术与变性梯度凝胶电泳(DGGE)技术,解析了石油污染土壤中细菌群落结构特征。结果表明,在总石油烃的质量分数高于30 mg/g(以干土计)的重度污染区域,井距相同的土样中的细菌群落表现出对石油污染胁迫较为一致的响应,某些乳球菌(Lactococcus sp.)和无色细菌(Achromobacter sp.)等细菌相对数量显著增加,可以判断其对石油烃的降解起着重要作用。通过对细菌群落结构的解析,可以为石油污染土壤的微生物修复提供理论依据。PCR-DGGE was used to investigate the structure of bacterial community in oil contaminated soil.The results showed that the bacterial community responded remarkably to the environmental stress in the heavy petroleum hydrocarbons polluted area with a content of 30mg/g（based on the mass of dry soil）.And the relative quantity of some members of genera Lactococcus sp.and Achromobacter sp.remarkably increased,which might play an important role in the oil degradation.The analysis of bacterial community structure would provide crucial foundation in the theory for microbial remediation of polluted soils.

关 键 词：石油烃 土壤污染 PCR-DGGE 细菌群落 

分 类 号：X172[环境科学与工程—环境科学] TE991[石油与天然气工程—石油机械设备]