反向被动血凝试验检测人胃液潜血的探讨  

The Study of Gastric Juice Occult Blood Test with Reverse Passive Hemagglutination(RPHA)

作　　者：杨本付[1,2] 王润田[1,2] 王涛[1,2] 

机构地区：[1]济宁医学院流行病学教研室 [2]北京医科大学流行病学教研室

出　　处：《中国慢性病预防与控制》1999年第2期54-55,共2页Chinese Journal of Prevention and Control of Chronic Diseases

摘　　要：目的：研究一种检测微量胃液潜血的新方法。方法：建立用反向被动血凝试验（ＲＰＨＡ）检测人胃液潜血的方法，检测正常人和胃癌及其癌前疾病等患者共１８５份胃液标本。结果：ＲＰＨＡ法检测血红蛋白（Ｈｂ）的最低浓度为０．６μｇ／ｍｌ，最低检出量为１５ｎｇ。以胃癌和不典型增生阳性率最高（１００％），其次是萎缩性胃炎（８４．６２％）。正常空腹胃液（ｐＨ１．５）对Ｈｂ的抗原性仅有轻度影响。结论：本ＲＰＨＡ法具有特异性强、敏感性高、方法简便、抗体用量少、无需特殊仪器、结果易观察等优点。Objective:To explore a new method to detect occult blood in gastric juice.Method:The RPHA was established in this study to examine occult blood in human gastric juice.185 samples of stomach cancers,precancerous conditions and normals were examined.Results:Minimum detectable hemoglobin(Hb) in hemolysate of the RPHA was 0 6 μg/ml.The patients with sto mach cancer and dysplasia had the highest positive rate(100%) of RPHA.The next is atrophic gastritis(84.62%).The normal fasting gastric juice(pH1.5) just had a slight effect on the antigenicity of Hb.Conclusion:The RPHA has a high specificity and sensitivity.The method is simple and easy to observe the endpoint.It just needs a few antibody without special instruments.It will be of some application value in the screening of stomach cancer and precancerous conditions.

关 键 词：血凝试验 胃液 潜血 胃肿瘤 

分 类 号：R735.204[医药卫生—肿瘤] R730.43[医药卫生—临床医学]