磁性壳聚糖、海藻酸钠固定β-呋喃果糖苷酶研究  

Research of β-fructofuranosidase immobilized by magnetic chitosan microspheres-sodium alginate

作　　者：杨秀芳[1] 陈梅[1] 马养民[1] 

机构地区：[1]教育部轻化工助剂化学与技术重点实验室陕西科技大学,陕西西安710021

出　　处：《粮食与油脂》2010年第11期21-24,共4页Cereals & Oils

基　　金：陕西省教育厅基金项目(08JK238)

摘　　要：分别以海藻酸钠、壳聚糖复合Fe3O4为载体,采用包埋―交联法固定β–呋喃果糖苷酶。对固定化过程氯化钙浓度、戊二醛浓度、加酶量、包埋时间、交联时间等因素进行考察;并采用正交试验对载体制备与酶固定化中主要条件进行优化;通过对固定化酶活力回收比较,磁性海藻酸钠固定化酶活力回收率优于磁性壳聚糖。β-fructofuranosidase was co-immobilized by embedding-crosslinking with sodium alginate and chitosan complexed with Fe3O4.Some factors that have effect on activity of immobilized β-fructofuranosidase were studied.Such as the concentration of Calcium chloride,the density of glutaraldehyde,amount of β-fructofuranosidase,embedding time,crosslinking time and so on.The main conditions were finally optimized and determinated by the orthogonal experiments.Magnetic sodium alginate is better than magnetic chitosan,by comparing of yield of immobilized enzyme

关 键 词：磁性壳聚糖微球 磁性海藻酸钠 β–呋喃果糖苷酶 

分 类 号：TQ426.97[化学工程]