Calbindin D28k对帕金森病模型小鼠纹状体凋亡相关蛋白表达的影响  

Effects of calbindin D28k on expression of apoptosis-associated proteins in the striatum of the mouse model of Parkinson's disease

作　　者：孙申[1] 高秀先[1] 刘美英[1] 袁红花[1] 高殿帅[1] 

机构地区：[1]徐州医学院神经生物学研究中心,221004

出　　处：《中华老年心脑血管病杂志》2010年第12期1125-1128,共4页Chinese Journal of Geriatric Heart,Brain and Vessel Diseases

基　　金：国家自然科学基金(30570564);教育部科学技术研究重点项目(204055)

摘　　要：目的观察1-甲基4-苯基1,2,3,6四氢吡啶(MPTP)致帕金森病(PD)模型小鼠黑质多巴胺能神经细胞过表达Calbindin D28k(CB)时,纹状体细胞抗损伤作用机制。方法选择C57BL/6小鼠连续5 d腹腔注射MPTP,构建成功PD模型小鼠30只,随机分为模型组,人类免疫缺陷病毒(HIV)Ⅰ组(注射HIV Ⅰ)和CB-HIV-Ⅰ组(注射CB-HIV-Ⅰ),每组10只,连续6周对各组小鼠行为学检测,Western blot法检测各组小鼠CB,Bcl 2和Bax的表达变化。结果与模型组和HIV-Ⅰ组比较,CB-HIV-Ⅰ组小鼠各时间点移动格子次数,第1、2、5和6周站立次数,第6周时游泳和悬挂时间,差异有统计学意义(P<0.05,P<0.01);CB-HIV Ⅰ组小鼠中脑黑质中CB的表达量显著升高(P<0.05),纹状体细胞中Bcl-2的表达量亦明显升高(P<0.01),而Bax的表达量明显降低(P<0.01)。模型组和HIV-Ⅰ组上述指标差异无统计学意义(P>0.05)。结论黑质多巴胺能神经细胞过表达CB时,纹状体细胞Bcl-2/Bax表达上调,提示其与纹状体细胞抗凋亡能力增强有关。Objective To study the effect of over-expression of calbindin D28k（CB） in substantia nigra dopaminergic neurons on the expression of Bcl-2 and Bax in the striatum of 1-methyl-4-phe- nyl-1,2,3,6-tetrahydropyridine（MPTP）-induced mouse model of Parkinson＇s disease（PD） and to discover CB＇s neuroprotective mechanism at protein expression levels. Methods C57BL/6 mice were injected intraperitoneally with MPTP for 5 days to establish 30 mouse models of PD. Then, the mice were randomly divided into 3 groups（10 mice/group）：control group, HIV-Ⅰ group（in- jected with HIV-Ⅰ ）, and CB-HIV-Ⅰ group（injected with CB-HIV-Ⅰ ）. The Western blot was used to detect the expression levels of CB,Bcl-2 and Bax. Results Compared with control group and HIV-Ⅰ group,the expression of CB and bcl-2 increased （P 〈 0.05,P〈 0.01） ,while the ex- pression of bax decreased （P 〈 0.01） in CB-HIV-Ⅰ group. Conclusion The over-expression of CB in substantia nigra＇s dopaminergic neurons may promote striatum cells＇ resistance to apoptosis,which is related to the increase in Bcl-2/Bax ratio.

关 键 词：帕金森病 细胞凋亡 钙结合蛋白 维生素D依赖性 基因 BCL-2 BCL-2相关X蛋白质 CALBINDIN D28k 

分 类 号：R742.5[医药卫生—神经病学与精神病学]