阿尔茨海默病APPswe/PS1dE9双转基因型与野生型小鼠学习记忆能力的比较  

Comparison of Learning and Memory of APPswe/PS1dE9 Double Transgenic Mice Model of Alzheimer’s Disease and Wild-type Mice

作　　者：赵燕星[1] 苏殿三[1] 王祥瑞[1] 陈杰[1] 

机构地区：[1]上海交通大学医学院附属仁济医院麻醉科,200127

出　　处：《中国临床神经科学》2010年第6期561-564,共4页Chinese Journal of Clinical Neurosciences

基　　金：国家自然科学基金(30801069);上海市卫生局(2006Y027);上海市教委(08YZ36);上海市自然科学基金(08ZR1413700);教育部博士点基金(200802481094);上海市科技启明星(09QA1403800);上海交通大学医学院重点学科

摘　　要：目的:比较不同年龄段阿尔茨海默病(AD)APPswe/PS1dE9双转基因型与野生型小鼠的学习记忆能力。方法:C57BL/6J雌鼠与C3H/HeJ雄鼠交配产下F1代,再将F1代与3月龄的APPswe/PS1dE9双转基因型小鼠交配产下F2代。提取F2代小鼠鼠尾DNA,PCR扩增目的基因并鉴定。依据是否含APP和PS1基因分为转基因型组和野生型对照组,小鼠分别于6、8月龄行Morris水迷宫实验,11月龄行Y迷宫实验检测学习记忆能力。结果:繁育F2代小鼠33只,其中转基因型组18只,野生型对照组15只。6月龄转基因型与野生型小鼠定位航行实验潜伏期的差异无统计学意义,空间探索实验目标象限时间和路程百分比的差异无统计学意义(P>0.05)。与8月龄野生型小鼠比较,转基因型小鼠定位航行实验第1天潜伏期延长(P<0.05)。与11月龄野生型小鼠比较,转基因型小鼠达到学会标准的训练次数增加(P<0.05)。结论:ADAPPswe/PS1dE9双转基因型与野生型小鼠比较,于11月龄时出现学习记忆能力障碍。To compare learning and memory of APPswe/PS1dE9 double transgenic mice of Alzheimer’s disease（AD） and wild-type mice at the stage of different age. Methods： Female C57BL/6J mice were mated with male C3H/HeJ mice and the first filial generation（F1） were gotton. Then F1 mice were mated with 3 month-old APPswe/PS1dE9 double transgenic mice to get F2. DNA of each pup were extracted from the tails of F2 mice and amplified by polymerase chain reaction （PCR）. According to the results of PCR, F2 mice were classified into transgenic group and wild-type group. Learning and memory were assessed by Morris water maze at the age of 6 or 8 months respectively and by Y-maze at 11 months. Results： Eighteen APPswe/ PS1dE9 double transgenic mice were identified from thirty three pups. At 6months, no significant statistical difference was found about the mean latency in place navigation test of APPswe/PS1dE9 double transgenic mice and wild-type mice, nor were the percentage of time and distance spent in the target quadrant in probe trials（P〉0.05）. At 8 months, compared with the wild-type, the mean latency in place navigation test of APPswe/PS1dE9 double transgenic mice was prolonged in the first day （P〈0.05）. At 11 months, the time of training to learn standard of APPswe/PS1dE9 double transgenic mice increased（P0.05）. Conclusion： APPswe/PS1dE9 double transgenic mice had dysfunction of learning and memory about at the age of 11 months.

关 键 词：学习记忆 APPswe/PS1dE9双转基因小鼠 阿尔茨海默病 Y迷宫 MORRIS水迷宫 

分 类 号：R-055[医药卫生]