嗜肿瘤Ⅰ型单纯疱疹病毒介导GALV fus基因治疗肺腺癌的体外试验研究  

Antitumor Therapy with GALV Membrane Fusion Glycoprotein Mediated by Type I Herpes Simplex Virus for Lung Adenocarcinoma in vitro

作　　者：朱冰[1] 杨建茹 江跃全[1] 陈诗奉[1] 付新平[1] 

机构地区：[1]重庆医科大学第二附属医院胸心外科,重庆市400010 [2]邯郸市第六医院中心实验室

出　　处：《中国肿瘤临床》2010年第22期1261-1264,共4页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金(编号:30471984);重庆市卫生局医学科研项目(编号:06-2-001);医学科研计划项目基金资助(编号:2010-02-127)~~

摘　　要：目的:探讨特异性启动子UL38及无肿瘤特异性巨细胞病毒启动子(CMVP)调控的嗜肿瘤Ⅰ型单纯疱疹病毒(HSV-Ⅰ)介导的长臂猿白血病病毒致融性外膜糖蛋白(GALV.fus)基因系统体外对人肺腺癌的选择性杀伤效应。方法:选择非洲绿猴肾细胞(Vero),肺腺癌细胞(A549)和正常人胚胎成纤维细胞(HFL-Ⅰ GNHu 5),将构建成功的重组HSV-Ⅰ载体通过脂质体转染、导入包装细胞Vero中制备重组HSV-Ⅰ病毒,用来转染A549和HFL-Ⅰ GNHu 5,观察其转染、表达状况及体外抗肿瘤作用,增强型绿色荧光蛋白(EGFP)基因片段取代GALV.fus作对照质粒。提取受染A549和HFL-Ⅰ GNHu 5细胞的总蛋白,以Western Blot法鉴定GALV.fus基因的表达。结果:成功包装重组HSV-Ⅰ载体,致融性病毒感染A549后引起强烈的细胞膜融合,且杀伤作用随着感染剂量的增加而增强。而非致融病毒仅引起病毒细胞毒性效应,细胞呈圆形,镜下未见细胞融合斑。CMVP调控的重组HSV-Ⅰ在HFL-ⅠGNHu 5生长静止抑制状态均引起细胞膜融合,UL38P调控的重组HSV-Ⅰ仅在生长状态导致细胞膜融合;而细胞处于静止和抑制状态时,不引起细胞膜融合以及任何细胞病理改变。以Western Blot法检测受染A549和HFL-Ⅰ GNHu 5细胞,均可检到GALV.fus基因蛋白表达。结论:UL38p调控的GALV.fus基因在有效的载体内对体外肺癌细胞有强效的选择性杀伤效果和应用安全性,为临床肺癌治疗探索出一种新型基因治疗方法。Objective： To observe the selective killing effect of a recombinant Type I Herpes simplex virus （HSV-I）-mediated Gibbon ape leukemia virus membrane fusion glycoprotein （GALV.fus） gene system controlled by UL38 and cytomegalovirus （CMV） promoters on lung adenocarcinoma in vitro. Methods： Lung adenocarcinoma cell line A549, human fetal fibroblast cell line HFL-I GNHu5 and Vero green monkey kidney cells were cultured for study. Recombinant HSV-I plasmids encoding GALV.fus were introduced into Vere cells by liposome to amplify the virus, and then the virus was transfected into A549 and HFL-I GNHu 5 cells. The expression of the recombinant plasmids and antitumor effect in vitro were observed. Recombinant cytomegalovirus （CMV） containing GALV.fus or enhanced green fluorescence protein was used as the control. Total protein was extracted from the transfected A549 and HFL-I GNHu5 cells, and expression of the GALV.fus gene was detected by Western Blot. Results： Recombinant HSV-I virus was packed successfully. The GALV.fus gene system caused A549 cells to form syncytia in vitro and showed increasing cell death with increasing multiplicity of infection. On the other hand, infection with the control virus induced a typical cytopathic effect, characterized by the cells becoming rounder and swelling. HSV Synco-2 was tested for loss of its ability to cause syncytial formation in normal non-dividing human cells by infecting primary human fibroblasts in either a quiescent or a cycling state. Infection with recombinant HSV-I mediated GALV.fus gene system caused syncytial formation in these normal human cells when they were cycling. CMV promoter-me-diiated cell fusion was only marginally affected in cells whose cycling was either slowed by serum starvation or completely arrested with Iovastatin. UL38 promoter-mediated cell fusion, on the other hand, was absent in cells whose cycling was decreased or arrested. By Western Blot, the expression of GALV.fus gene could be detected in both A549 cells and embryonic fi

关 键 词：长臂猿白血病病毒致融性外膜糖蛋白 I型单纯疱疹病毒 肺腺癌 基因治疗 

分 类 号：R734.2[医药卫生—肿瘤]