人内源性逆转录病毒K包膜蛋白在乳腺肿瘤组织中的表达及意义  

The Expression of Human Endogenous Retrovirus K Envelope Protein in Breast Tumor and Its Significance

作　　者：耿姗姗[1] 赵晶[1] 顾林[1] 许旭[1] 刘红[1] 

机构地区：[1]乳腺癌防治教育部重点实验室,天津市肿瘤防治重点实验室,天津医科大学附属肿瘤医院乳腺二科,天津市300060

出　　处：《中国肿瘤临床》2010年第22期1286-1289,共4页Chinese Journal of Clinical Oncology

基　　金：天津市自然科学基金项目资助(编号:10JCZDJC20100)~~

摘　　要：目的:检测人内源性逆转录病毒K(HERV-K)包膜蛋白(env蛋白)在不同乳腺肿瘤组织中的表达,分析其表达水平与乳腺癌患者临床病理特征的相关性,探讨HERV-K env蛋白的表达与乳腺癌预后的关系。方法:免疫组织化学法检测HERV-Kenv蛋白在95例乳腺癌组织、30例乳腺良性肿瘤组织中的表达情况,x^2检验用于分析HERV-K env蛋白的表达水平与乳腺癌患者临床病理特征的相关性。结果:免疫组织化学结果显示,HERV-K env蛋白的阳性表达主要位于细胞浆,该蛋白在乳腺癌组织中多呈强阳性或中度阳性表达,而在乳腺良性肿瘤组织中多呈阴性或弱阳性表达。HERV-K env蛋白在乳腺癌组织中的阳性表达率(88.4%)明显高于乳腺良性肿瘤组织(40.0%),差异具有统计学意义(P<0.001)。HERV-K env蛋白的表达水平与乳腺癌的某些预后因素存在相关性。在HERV-K env蛋白表达阳性的乳腺癌患者中,该蛋白的表达水平与肿瘤大小、临床分期、腋窝淋巴结转移数目之间存在正相关关系,与ER的表达状况之间存在负相关关系(P均<0.05),而与患者的年龄、组织学分级以及PR的表达状况无相关性(P均>0.05)。结论:HERV-K env蛋白在乳腺良性肿瘤中多为阴性表达,而在乳腺癌中多呈阳性表达,并且可能与乳腺癌的发展及预后相关,该蛋白在乳腺癌中的表达水平可能是一种判断乳腺癌预后的指标。Objective： To detect the expression of human endogenous retrovirus K （HERV-K） envelope （env） protein in breast tumors; to analyze the association between the expression level of HERV-K env protein and clinicopathologic characteristics of breast cancer; to explore the relationship between HERV-K env protein expression and the prognosis for patients with breast cancer. Methods： Immunohistochemistry was used to detect the expression of HERV-K envelope protein in 95 cases of breast cancer and 30 cases of benign breast tumor tissue. The X2 test was used to analyze the relationship between the level of expression and clinicopathological characteristics. Results： Immunohistochemical staining results revealed that the positive expression of HERV-K env protein was evident in the cytoplasm. Breast cancer tissue showed medium or high expression in almost all cells. However, in benign breast tumor tissue the expression level was low or negligible. The positive expression rate of HERV-K env protein was 88.4% （84/95） in breast cancer and 40.0% （12/30） in benign breast tumor tissues. The expression of HERV-K env protein in breast cancer was significantly higher than that in benign breast tumor tissue （P〈0.001）. The expression level of HERV-K env protein was correlated with some prognostic factors. Among the patients with breast cancer who had positive expression of HERV-K env protein, the expression level had a posi- tive correlation with tumor size, clinical stage and the number of positive axillary nodes, whereas, it had a negative correla- tion with the protein expression of ER （P〈0.05）. No correlation was found between its expression level and age, histological grade, or the protein expression of PR in the patients with breast cancer （/〉〉0.05）. Conclusion： The expression of HERV-K env protein was negative in most benign breast tumors, but positive in almost all malignant breast tumors. Thus it may be correlated with the development and prognosis of breast cancer. The expression le

关 键 词：人内源性逆转录病毒(HERV-K) 乳腺肿瘤包膜(env)蛋白 免疫组织化学法 

分 类 号：R737.9[医药卫生—肿瘤]