Survivin在喉鳞癌中的表达及其与肿瘤细胞增殖和凋亡的关系  

Expression of survivin and its correlation with proliferation and apoptosis in laryngeal squamous cell carcinoma

作　　者：李大伟[1] 董频[1] 孙臻峰[1] 徐宏鸣[1] 王国良[1] 

机构地区：[1]上海交通大学附属第一人民医院耳鼻喉头颈外科,上海200080

出　　处：《临床肿瘤学杂志》2010年第11期966-969,共4页Chinese Clinical Oncology

基　　金：上海市生物医药领域重点科技项目(09411951000)

摘　　要：目的分析Survivin在喉鳞癌中的表达及其与肿瘤细胞增殖、凋亡的关系,并探讨其与喉鳞癌临床病理特征之间的关系。方法应用免疫组化染色技术检测Survivin和Ki-67在86例喉鳞癌组织及32例正常喉黏膜上皮组织中的表达;TUNEL法检测喉鳞癌细胞的凋亡情况。结果 Survivin在喉鳞癌组织中阳性表达率为60.5%,高于正常喉黏膜上皮组织的12.5%(P<0.05),Survivin阳性表达率与肿瘤临床分期、病理分级、淋巴结转移有关(P<0.05);喉鳞癌Survivin阳性组中凋亡指数(AI)低于阴性组(P<0.05),增殖指数(PI)高于阴性组(P<0.05),且Survivin表达与AI呈负相关(r=-0.831,P<0.05),与PI呈正相关(r=0.883,P<0.05)。结论 Survivin在喉鳞癌中高表达,可能与喉鳞癌细胞的增殖和凋亡均有一定的关联。Objective To analyze the relationship between survivin protein expression and cell proliferation as well as apoptosis in human laryngeal squamous cell carcinoma（ LSCC）, and evaluate their clinicopathological significances. Methods Immuohistochemical staining technique was taken to detect the expression of survivin and Ki-67 and TUNEL was taken for apoptosis in 86 LSCCs and 32 laryngeal epithelium mucosa tissues. Results In LSCCs, the positive expression rate of survivin was 60. 5% , which was higher than that in laryngeal mucosa tissues（P 〈0. 05 ）. The positive expression rate of survivin was correlated with TNM stage, histological grade and lymph node metastasis （P 〈 0. 05 ）. The apoptotic index （AI）in survivin positive group was significantly lower than that in survivin negative group （P 〈 0. 05 ）, and AI was negatively correlated with weighted survivin score（ r = -0. 831, P 〈 0. 05 ）. The proliferation index （PI） in survivin positive group was significantly higher than that in survivin negative group （ P 〈 0. 0_5 ）, and PI was positively correlated with weighted survivin score （ r = 0. 883, P 〈 0. 05 ）. Conclusion Survivin is overexpressed in LSCCs, and it may play important roles in human laryngeal tumorigenesis through the inhibition of apoptosis and acceleration of proliferation activity.

关 键 词：喉肿瘤 鳞状细胞癌 SURVIVIN蛋白 增殖 凋亡 

分 类 号：R739.65[医药卫生—肿瘤]