人小细胞肺癌多药耐药细胞H446/CDDP基因组DNA甲基化状态的变化  

Genomic DNA methylation in multidrug-resistant cell line H446/CDDP of human small-cell lung cancer

作　　者：郭芮伶[1] 吴国明[2] 李海东[1] 潘峰[1] 李文碧[1] 戢福云[2] 钱桂生[2] 

机构地区：[1]解放军第324医院呼吸内科,重庆400020 [2]第三军医大学新桥医院全军呼吸内科研究所,重庆400037

出　　处：《第三军医大学学报》2010年第23期2533-2535,共3页Journal of Third Military Medical University

基　　金：成都军区医学科研计划课题(MB09010)~~

摘　　要：目的探讨基因组DNA甲基化状态与人小细胞肺癌(small cell lung cancer,SCLC)H446/CDDP细胞获得性多药耐药的关系。方法分别提取人SCLC多药耐药细胞H446/CDDP及其亲代细胞H446的基因组DNA,应用甲基化敏感性随机引物PCR(MS-AP-PCR)方法检测其甲基化状态。结果 H446/CDDP细胞经酶解后的扩增谱带较H446细胞明显增多,且荧光强度较强,表明其基因组DNA甲基化水平较亲代细胞明显增高。结论 H446/CDDP细胞多药耐药性的获得可能与其基因组DNA甲基化水平增高有关。Objective To study the correlation between genomic DNA methylation and acquired multidrug-resistance of human small cell lung cancer （SCLC） cell line H446/CDDP. Methods Genomic DNA was extracted from H446/CDDP cells and their parental cells H446. Genomic DNA methylation was detected by methylation-sensitive arbitrarily primed PCR （MS-AP-PCR）. Results The amplified bands of H446/CDDP cells were significantly more than those of H446 cells and their fluorescence intensity was higher than that of H446 cells after enzymolysis, indicating that the genomic DNA methylation level is higher in H446/CDDP cells than in H446 cells. Conclusion The acquired multidrug-resistance of H446/CDDP cells is correlated with the high genomic DNA methylation level.

关 键 词：人小细胞肺癌 多药耐药 DNA甲基化 甲基化敏感性随机引物PCR法 

分 类 号：R394.2[医药卫生—医学遗传学] R733-54[医药卫生—基础医学]