黄鳝体表溃疡病病原菌的分离与鉴定  

ISOLATION, IDENTIFICATION AND PATHOGENICITY OF CAUSATIVE PATHOGEN FOR MUSCULAR ULCER IN MONOPTERUS ALBUS

作　　者：任红梅[1] 何智[1] 杨德英[2] 杨光友[2] 古小彬[2] 王淑贤[2] 

机构地区：[1]四川农业大学动物科技学院水产系,雅安625014 [2]四川农业大学动物医学院预防兽医系,雅安625014

出　　处：《水生生物学报》2010年第6期1106-1112,共7页Acta Hydrobiologica Sinica

基　　金：教育部<长江学者和创新团队发展计划>创新团队项目(IRTO848)资助

摘　　要：研究首次报道从患体表溃疡病黄鳝的肝脏、肾脏、脾脏等分离到1株细菌,经动物回归试验鉴定其为病原菌。形态学观察、生理生化试验和16S rRNA基因鉴定,结果证实该病原菌株为温和气单胞菌(Aeromonas sobria)。通过致病性试验证明其对鲤鱼和泥鳅均有致病力;药敏试验结果表明:分离菌株对头孢氨噻肟、头孢曲松钠、丙氟哌酸、洛美沙星、甲氧苄啶、氟哌酸、壮观霉素等高度敏感,而对氨苄青霉素、乙酰螺旋霉素等药物不敏感。Rice field eel,Monopterus albus,freshwater fish,belongs to the synbranchidae.It is an economically important species for fish production in China,especially in southern of China.The diseases of cultured rice field eel have been the bottle-neck for the sustainable development of rice field eel cultivation.The muscular ulcer disease is a kind of common disease to rice field eel and is suggested to be one of the most important diseases in industrial artificial-breeding.In 2007,the outbreak of muscular ulcer disease in the rice field eel farmed in Sichuan province,which was high incidence and mortality and caused lots of economical loss to the farmer.To make certain the causative pathogen,we studied the etiology and virulence of rice field eel so as to provide basic data for the farming industry of rice field eel.In the present study,we used the morphological,physiological and biochemical methods to identify the isolated bacterium.Meanwhile,to ensure the results accurately,the phylogenetic analyses were also used.Liver,kidney and spleen of diseased rice field eel were aseptically streaked on Nutrient Agar by swabbing.After incubation,pure cultured colonies were obtained and one causative bacteria（hs-1） was isolated from liver,kidney and spleen of moribund rice field eel.The characteristics of causative bacteria were tested by bacteria micro amount biochemical fermentation tube,Gram＇s staining and another method.The phylogenetic comparison based on 16S rRNA gene of the causative bacterium was also studied,the amplified nucleotide sequence of isolated bacterium（GenBank accession No.FJ461353） had 99% homology with the other 16S rRNA sequences of A.Sobria.Based on the morphological,physiological and biochemical characteristics,as well as the results of phylogenetic analysis,the causative bacterium was conducted as A.Sobria.Pathogenicity tests in rice field eel showed that the symptoms of rice field eel were similar with the natural diseased eels.The mortality of high dose injected group（3.0×108 CFU） was 10

关 键 词：黄鳝 温和气单胞菌 分离鉴定 致病性 

分 类 号：S941[农业科学—水产养殖]