鹅ACSL1基因克隆及其在鹅肥肝形成中作用的初步研究  

Cloning of Goose ACSL1 Gene,Tissues Expression and the Effect of Overfeeding on Its mRNA Level

作　　者：潘志雄[1] 吕佳[1] 卢立志[2] 王继文[1] 

机构地区：[1]四川农业大学畜禽品种资源发掘与利用重点实验室,雅安625014 [2]浙江省农业科学院畜牧兽医研究所,杭州310021

出　　处：《畜牧兽医学报》2010年第11期1407-1413,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家水禽产业技术体系(nycytx-45-05)

摘　　要：本研究旨在克隆鹅长链酯酰辅酶A合成酶1(ACSL1)基因,并研究其在填饲诱导鹅肥肝形成中的作用,为揭示ACSL1在鹅肥肝形成过程中的作用提供依据。以前期抑制消减杂交文库筛选的部分ESTs序列为基础,通过RT-PCR扩增鹅ACSL1基因CDS、并用实时定量等技术检测该基因在皮脂、腹脂、肝脏等10个组织中的表达情况及填饲对其在肝脏中表达的影响,同时与填饲后鹅肝脏总脂质、甘油三酯(TG)、肝质量等指标进行相关分析。结果发现:鹅ACSL1基因CDS全长2100bp,编码699个氨基酸。保守结构区预测发现,其蛋白质跟其他物种一样,也存在2个保守功能区(ATP/AMPmotif和FACSmotif)和1个跨膜结构域,它与鸡、牛、人、小鼠ACSL1基因的同源性分别为93%、80.4%、78.5%、77.3%;该基因主要表达于腹脂、皮脂、肝脏,而在其他组织表达相对较低;填饲能引起ACSL1mRNA在鹅肝脏的表达丰度极显著增加(P<0.01),且与肝质量、肝内TG和总脂质呈显著正相关;同时,填饲导致其在朗德鹅肝脏中的表达丰度显著高于四川白鹅(P<0.05)。结果提示:填饲引起ACSL1mR-NA在鹅肝脏中极显著增加(P<0.01),且其增加幅度存在着品种间差异(P<0.05)。This experiment was conducted to study the effect of overfeeding on ACSL1 mRNA level in goose.In this study,the Landes geese and Sichuan White geese were used to clone the gene sequence of ACSL1,and the effect of overfeeding on the transcriptional level of ACSL1 in liver was researched.The result indicated that the obtained CDS sequence of ACSL1 gene was 2 100 bp,the sequence analysis revealed that its open reading frame（ORF） encoding 699 amino acids,which contain a ATP/AMP motif and a FACS motif,and has high homology with the corresponding sequences of chicken（93%）,cattle（80.4%）,human（78.5%） and mouse（77.3%）,respectively.The expression of ACSL1 was measured in several tissues,and the effects of overfeeding on the expression of ACSL1 were studied.The results of real time RT-PCR demonstrated that,compared to the other tissues,goose ACSL1 mRNA was more abundant in abdominal adipose tissues,subcutaneous adipose tissues and liver.Overfeeding markedly increased the mRNA expression of ACSL1 gene in the liver of two breeds,and gene expression was markedly higher in Landes geese than that in Sichuan White geese.The mRNA abundance of ACSL1 was positively correlated to the relative weight of liver,TG levels and total of fatty acids after overfeeding,and the correlation in Landes geese was stronger than that in Sichuan White geese.It was concluded that overfeeding induced the significant increase of ACSL1 mRNA level in goose liver,and the effect of overfeeding was different between the two breeds.

关 键 词：鹅 填饲 ACSL1 基因克隆 MRNA表达 

分 类 号：S835[农业科学—畜牧学] Q756[农业科学—畜牧兽医]