脂肪干细胞构建组织工程化角膜基质组织  

Tissue engineering of corneal stroma with adipose derived stem cells

作　　者：鲍慧婧[1] 邹俊[1] 尹烁[2] 崔磊[2] 

机构地区：[1]上海交通大学附属第六人民医院眼科,上海200233 [2]上海组织工程研究与开发中心,上海200235

出　　处：《复旦学报（医学版）》2010年第6期631-636,共6页Fudan University Journal of Medical Sciences

基　　金：上海市自然科学基金项目(08ZR1411400)

摘　　要：目的探讨以可降解高分子材料聚羟基乙醇(polylactic-co-glycolic acid,PLGA)为支架,复合自体脂肪干细胞构建组织工程角膜基质修复角膜基质层缺损的可行性。方法兔脂肪干细胞(rabbit adipose derivedstem cells,rASCs)培养至第4代接种在PLGA载体支架上,扫描电镜观察细胞在支架上生长黏附情况。体外培养7天后将rASCs-PLGA复合物回植到角膜基质缺损模型的兔角膜基质层间,术后第12周和24周取材行组织学和透射电镜超微结构观察。未接种细胞的PLGA材料移植基质层间作为对照组。结果细胞在PLGA支架上生长增殖良好,实验组移植术后12周材料降解,角膜基本恢复透明。组织学检查显示移植后24周新生角膜基质样组织与正常角膜基质组织相似,电镜下胶原纤维直径与正常角膜基质组织比较,差异无统计学意义(P>0.05)。结论脂肪干细胞可作为种子细胞来源用于构建组织工程角膜基质。Objective To explore the feasibility of using autologous rabbit adipose derived stem cells（rASCs） for corneal stromal defect repairing with biodegradable polylactic-co-glycolic acid（PLGA） as scaffold.Methods ASCs isolated from rabbit lipoaspirate were cultured to passage 4 and seeded on PLGA to fabricate cell-PLGA constructs.Scanning electron microscope was used to observe the adhesion and proliferation of cells on scaffold.After 1 week of cultivation in vitro,the construct was implanted into rabbit corneal stroma.The tissue engineered cornea was observed and harvested for histological evaluation and transmission electron microscopy（TEM） examination at 12 weeks and 24 weeks after transplantation.The rabbits implanted with PLGA scaffold alone without cells were served as control.Results rASCs attached firmly and grew well on the scaffold in vitro.The PLGA material was degraded and the cornea was nearly transparent at 12 weeks after transplantation in vivo.Histological structure of tissue engineered cornea was relatively similar to that of normal cornea at 24 weeks after surgery.The diameter of collagen fibrils observed by transmission electron microscopy showed no significant difference with that of normal stroma.Conclusions Autologous rabbit adipose derived stem cells（rASCs） could be seed cells for constructing tissue engineered corneal stroma.

关 键 词：脂肪干细胞 组织工程 角膜 基质 聚羟基乙醇 兔 

分 类 号：Q813.1[生物学—生物工程] R779.65[医药卫生—眼科]