犬脊柱放疗对脊髓血管形态及血流量的影响  

Effects of radio therapy for vertebral on vascular morphology and blood flow volume of beagle spinal cord

作　　者：徐万龙[1] 锡林宝勒日 白靖平[1] 

机构地区：[1]新疆医科大学附属肿瘤医院骨与软组织肿瘤科,乌鲁木齐830011

出　　处：《重庆医学》2010年第23期3168-3169,3172,F0002,共4页Chongqing medicine

基　　金：新疆维吾尔自治区科技攻关(含重大专项)和重点项目(200833116)

摘　　要：目的观察成年比格犬脊柱放疗对脊髓血管形态及血流量的影响。方法以随机数字表法将24只比格犬随机分为两组,其中空白对照组8只,放疗组16只。在10-14 d内,对放疗组比格犬胸9-10椎体给予平均总剂量为60 Gy的放疗。放疗结束3个月后,采用放射性生物微球技术对两组犬胸9-10椎体脊髓小血管血流量进行测定。将两组比格犬处死后,取各组相同部位、相同位置脊髓,用透视电镜观察两组犬血管超微结构变化;血管免疫组化染色后,测量脊髓血管平均管径和平均截面积。结果放疗组脊髓组织的血管平均直径、平均截面积和血流量均显著低于空白对照组,差异有统计学意义（P〈0.05）。透射电镜观察显示放疗组的脊髓血管形态、内皮细胞超微结构同空白对照组相比均有明显的病理改变。结论放疗可影响脊髓血管形态及血流量,这可能是放疗所致脊髓功能障碍的重要因素之一。Objective To observe the ultrastructure of the blood vessel and the blood flow in beagle spinal cord after radiotherapy.Methods Select purebred beagles and 24 were divided into 2 groups randomly.Mimic a beagle clinical model of tumor in the ninth and tenth thoracic vertebrae.Then the second group beagles were irradiated by the radiotherapy,the total irradiation doses were 60 Gy in 10～14 d.Measured the blood flow in the spinal cord injury by the radiolabelling microsphere technique on third month after radiation.The samples of spinal cord were taken out from the same locum of the nine and tenth thoracic vertebrae.All the samples were observed by the electron microscope and evaluated by vessel staining assay.Results The results showed that the average diameter,average sectional area and the blood blow of the radiotherapy group were all lower than the blank group;The configuration and ultrastructure of vessels were also different between two groups.Conclusion The radiotherapy can affect the ultrastructure of the blood vessel and the blood blow in beagle spinal cord,which played an important role in the pathophysiological process of spinal cord injury induced by the radiotherapy.

关 键 词：放疗 脊髓 血管 放射性生物微球技术 

分 类 号：R730.5[医药卫生—肿瘤]