脑梗死患者血清NO及tNOS、iNOS的测定  

Determination of serum nitric oxide (NO) and nitric oxide synthesis (tNOS,iNOS) in the patients with cerebral infarction

作　　者：吴晓华[1] 闫金辉[1] 杨晓晖[1] 

机构地区：[1]沧州医学高等专科学校,河北沧州061001

出　　处：《中国医药导报》2010年第33期9-10,共2页China Medical Herald

基　　金：河北省2006年科学技术研究与发展指导计划项目(项目编号:062761569)

摘　　要：目的:通过观测正常人及脑梗死患者血清一氧化氮(NO)和一氧化氮合成酶(NOS)水平,探讨NO、NOS与脑梗死的关系。方法:用硝酸还原酶法测定血清NO。用NOS催化L-Arg和分子氧反应生成NO,NO与亲核性物质生成有色化合物后,再采用比色的方法测定血清NOS。测定42例脑梗死患者及18例健康人血清NO、tNOS和iNOS含量,分析上述指标的变化情况。结果:与健康对照组比较,脑梗死组NO含量明显降低(P<0.05);tNOS及iNOS含量无显著性差异(P>0.05)。结论:血清NO、tNOS和iNOS参与了脑梗死的病理生理过程,其测定有助于对脑梗死患者的诊断及康复治疗。Objective：By observing serum nitric oxide（NO） and nitric oxide synthesis（NOS） levels of normal subjects and patients with cerebral infarction,to explore the relationship between NO,NOS and cerebral infarction.Methods：The levels of serum NO were determined by nitrate reductase.And with NOS catalytic L-Arg and molecular oxygen response to generate NO,NO and pro-nuclear substances to conduct a colored compound,then with colorimetric method determination of serum NOS.In 42 patients with cerebral infarction and 18 healthy volunteers,serum NO,tNOS and iNOS contents were determined and analysis of changes in these indicators.Results：Compared with the healthy control group,the serum NO decreased significantly in infarction group（P0.05）;tNOS and iNOS was no significant difference（P0.05）.Conclusion：Serum NO,tNOS and iNOS have involved in the pathophysiology of cerebral infarction,the determination could help the diagnosis of cerebral infarction and rehabilitation.

关 键 词：脑梗死 一氧化氮(NO) 一氧化氮合成酶(NOS) 

分 类 号：R743[医药卫生—神经病学与精神病学]