从植物乳杆菌全细胞转化液中分离纯化γ-氨基丁酸的工艺研究  

Study on Separation and Purification Technology of γ-aminobutyric Acid by Whole-cell Bioconversion

作　　者：张术聪 刘婷婷[1] 杨套伟[1] 夏海锋[1] 饶志明[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室江南大学生物工程学院,硕士研究生江苏无锡214122

出　　处：《食品与发酵工业》2010年第11期1-5,共5页Food and Fermentation Industries

基　　金：国家"863计划"(2007AA02Z207);国家自然科学基金(30970056);霍英东青年基金(121020)

摘　　要：前期筛选获得1株高效转化L-谷氨酸为γ-氨基丁酸(GABA)的植物乳杆菌(Lactobacillus plantarum)GB01-21,该菌以浓度200 g/L的L-谷氨酸为底物,发酵20 h左右,能以99%的摩尔转化率生产γ-氨基丁酸,产物γ-氨基丁酸的终浓度可达140 g/L左右。从全细胞转化液中分离纯化γ-氨基丁酸,着重对脱色工艺进行了研究,考察了温度、时间、pH和活性炭添加量对脱色效果的影响。通过单因素实验的基础上的正交实验分析,确定了脱色最佳工艺条件为粉末活性炭(150～200目)用量为1.5%,脱色温度70℃,pH值4.0,脱色时间40 min,γ-氨基丁酸转化液的脱色率高达98.42%,γ-氨基丁酸的保留率可达97.23%。随后对γ-氨基丁酸转化液进行初步分离纯化,最终测得γ-氨基丁酸的回收率89.4%,纯度为96.7%。A strain named Lactobacillus plantarum GB01-21 that can produce γ-aminobutyric （GABA） efficiently was sereened in our laboratory. During the 20 hours, 200 g/L L-Glu ean be transformed into 140g/L GABA. In this work γ-aminobutyrie was separated and purified from the whole-eell transforming solution. The deeolorizing teehnieal eonditions sueh as temperature, time, pH and amount of aetive carbon were studied. As a result, the optimum deeolorization eonditions were determined through single factor and orthogonal experiments as follows： activated carbon dosage 1.5% , deeolorization temperature 70 % , pH value 4.0 and deeolorization time 30 rain. Under these conditions, the deeolorization rate and reeovery rate of bioeonversion broth reaehed 98.42% and 97.23% , respectively. Finally, γ-aminobutyrie acid was isolated and purified from bioeonversion broth and the recovery rate and the purity of γ-aminobutyrie acid was 89.4% and 96.7% , respeetively. These findings pave the way for industrial produetion of GABA.

关 键 词：Γ-氨基丁酸 全细胞转化 脱色 分离纯化 

分 类 号：TQ921[轻工技术与工程—发酵工程]