TGF-β1在表面处理钛合金种植体骨界面表达变化的实验研究  

Expression of TGF-β1 on the interface of surface-prepared titanium implant and bone

作　　者：李振春 宋明宇 

机构地区：[1]大连市口腔医院口腔修复科,大连辽宁116021

出　　处：《中国实用口腔科杂志》2010年第11期676-679,共4页Chinese Journal of Practical Stomatology

基　　金：辽宁省教育厅A类项目(20122172)

摘　　要：目的通过检测转化生长因子-β1(TGF-β1)在种植体骨界面骨组织中的表达,评价表面处理钛合金、钛合金及不锈钢种植体的生物相容性。方法于2002年3月至2003年3月在中国医科大学组织学与胚胎学教研室随机将90只大鼠分为3组,每组30只,雌雄各半。分别将表面氧化处理后的六铝四钒钛(处理钛合金组)、未经特殊处理的六铝四钒钛(Ti6Al4V组)、不锈钢(316L合金组),植入大鼠的下颌骨内,于术后第1、2、3、4、6周取材,进行HE染色观察及TGF-β1免疫组化染色灰度值判读。结果 (1)HE染色光镜观察:处理钛合金组种植体成骨细胞排列紧密,新生骨较其余两组更致密。(2)TGF-β1免疫组化染色灰度值的比较:处理钛合金组高于Ti6Al4V组,Ti6Al4V组高于316合金组(均P<0.05)。结论表面氧化处理可增强Ti6Al4V骨界面TGF-β1的表达,提高其生物相容性。Objective To evaluate the biocompatibility of surface-prepared Ti6Al4V by examining expression of TGF-β1 on the interface of implant and bone.Methods Three kinds of materials including surface-oxidated/non-prepared Ti6Al4V and stainless steel were randomly implanted into mandibles of 90 rats,specimens were obtained 1,2,3,4 and 6 weeks after implantation,respectively.HE stain and immunohistochemistry were used to examine the interface of implant and bone.Results Osteoblasts arranged more closely together in surface-prepared Ti6Al4V group than the other groups by IE observation.In comparison of TGF-β1 immunohistochemistry expression,surface-prepared Ti6Al4Vnon-prepared Ti6Al4V stainless steel（P〈0.05）.Conclusion Surface oxidation may enhance TGF-β1 expression on the implant-bone interface and increase the biocompatibility of implant.

关 键 词：种植体 转化生长因子-Β1 免疫组化 生物相容性 

分 类 号：R78[医药卫生—口腔医学]