抑肽酶对实验性急性肝损伤模型小鼠肝组织的保护作用  

Protective effect of aprotinin on liver tissues in mice with acute liver injury

作　　者：王强 王心童 王虹蛟 孟威宏[3] 颜炜群[4] 任立群[5] 

机构地区：[1]解放军第461医院内二科,吉林长春130021 [2]吉林大学白求恩医学院 [3]沈阳军区总医院心血管内科,辽宁沈阳110015 [4]吉林大学再生医学科学研究所生物化学研究室,吉林长春130021 [5]吉林大学药学院实验药理与毒理学教研室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第6期1039-1042,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金资助课题(30300153)

摘　　要：目的:观察抑肽酶对实验性急性肝损伤小鼠肝组织超微结构改变的影响,探讨其对肝组织的保护作用。方法:建立小鼠急性四氯化碳(CCl4)肝损伤模型,分为正常对照组、模型组、甘利欣注射液组及抑肽酶小、中、大剂量组。各组小鼠取肝组织做超薄切片,醋酸双氧铀-柠檬酸铅双重染色,透射电镜下观察其肝组织超微结构的改变。结果:模型组肝细胞呈肿胀状,肝细胞核呈固缩状,胞质内粗面内质网减少、线粒体固缩、基质空化。抑肽酶小剂量组个别细胞呈固缩状,肝细胞胞质内可见少量粗面内质网和溶酶体,胆小管腔内微绒毛消失。抑肽酶中剂量组肝细胞体积稍大,肝细胞核仁明显,胞质内粗面内质网和线粒体,可见大量糖原聚集,胆小管腔内微绒毛较多。抑肽酶大剂量组肝细胞结构接近正常对照组,细胞体积较大,细胞界限清楚,肝细胞核呈圆形,胞质内粗面内质网和线粒体丰富,可见糖原颗粒。甘利欣注射液组肝组织超微结构改变与抑肽酶中剂量组相似。结论:腹腔注射抑肽酶对实验性小鼠急性肝损伤的肝组织超微结构具有明显的保护作用,随着抑肽酶剂量的增大肝组织超微结构改变明显减轻。Objective To observe the influence of aprotinin in ultrastructural changes of liver tissues in mice with experimental acute liver injury and explore its protective effect on liver tissues.Methods The mouse medels with carbon tetrachloride（CCl4）liver injury were established and the mice were divided into normal control group, model group,ganlixin injection groups and low,middle and high dose aprotinin groups.The tissue specimens were obtained from various groups,uranium oxide-acetate-citric acid-lead double staining was used to observe the changes of liver tissues under TEM.Results In model group,the liver cells were swelled with pyknotic nucleus,reduced intracytoplasmic rough endoplasmic reticulum,pyknotic mitochondria,and cavitation.In low dose aprotinin group, the individual cells were pyknotic with a few rough endoplasmic reticulum and lysosomes in liver cells,timidEndoluminal microvilli disappeared.In middle dose aprotinin group,the size of liver cells was a little larger,the liver cell nucleolar was evident with intracytoplasmic rough endoplasmic reticulum,mitochondria,numerous glycogen accumulation and more timid Endoluminal microvilli.In high dose aprotinin group,the liver cell structures were similar to those in control.The ultrasturctural changes in ganlixin injection group were similar to those in middle dose aprotinin group.Conclusion The intraperitoneal injection of aprotinin has significantly protective effect on the ultrastructures of liver tissues in acute liver injury mouse models.With the increase of aprotinin,the ultrastructural changes of liver tissues are markedly reduced.

关 键 词：抑肽酶 四氯化碳 急性肝损伤 小鼠 电镜 

分 类 号：R-332[医药卫生] R575.32