喹硫平和万拉法新对小鼠实验性应激及脑缺血致神经元损伤的作用  

Effects of quetiapine and venlafaxine on neuronal injury after cerebral ischemia and stress in mice

作　　者：房绍宽[1] 白晶[1] 吴江[1] 

机构地区：[1]吉林大学第一医院神经内科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2010年第6期1067-1070,1206,共5页Journal of Jilin University：Medicine Edition

基　　金：加拿大健康研究基金资助课题(MOP79132)

摘　　要：目的:研究喹硫平和万拉法新对小鼠实验性应激及脑缺血致神经元损伤的影响,阐明喹硫平和万拉法新对小鼠全脑缺血及应激的保护作用。方法:雄性ICR小鼠缺血前给予万拉法新或喹硫平腹腔注射14d,第15天闭塞双侧的颈总动脉。实验分为假手术组、假手术加应激组、缺血加应激组、喹硫平缺血加应激组及万拉法新缺血加应激组。采用免疫组织化学方法检测小鼠全脑缺血后NeuN阳性细胞数量的变化;采用尼氏染色检测全脑缺血后海马齿状回区神经元损伤情况。结果:与假手术组比较,假手术加应激组小鼠NeuN阳性细胞数量无明显变化(P>0.05);与假手术组和假手术加应激组比较,缺血加应激组小鼠NeuN阳性细胞数量减少(P<0.05);与缺血加应激组比较,万拉法新缺血加应激组小鼠NeuN阳性细胞数量无明显变化(P>0.05),喹硫平缺血加应激组NeuN阳性细胞数量增加(P<0.05)。与假手术组比较,假手术加应激组小鼠尼氏染色阳性细胞数量无明显变化(P>0.05);与假手术组或假手术加应激组比较,缺血加应激组小鼠尼氏染色阳性细胞数量减少(P<0.05);与缺血加应激组比较,万拉法新缺血加应激组尼氏染色阳性细胞数量无明显变化(P>0.05),喹硫平缺血加应激组尼氏染色阳性细胞数量增加(P<0.05)。结论:喹硫平能够减轻小鼠应激及脑缺血所致神经元损伤,对小鼠应激及全脑缺血具有保护作用。Objective To study the influence of quetiapine and venlafaxine in neuronal injury after cerebral ischemia and stress and illuminate the protective effects of quetiapine and venlafaxine after global cerebral ischemia and stress. Methods Male ICR mice were pretreated with venlafaxine or quetiapine for 14d.On the 15th day,the bilateral common carotid arteries of mice were occluded.This experiment was divided into sham group （Saline）,sham plus stress group （Saline＋Sham）,ischemia plus stress group（Saline＋Ischemia＋Stress）,quetiapine plus ischemia plus stress group（QUE＋Ischemia＋Stress）and venlafaxine plus ischemia plus stress group（VEN＋Ischemia＋ Stress）.The neuronal injury after cerebral ischemia and stress was detected with immunohistochemical and Nissle staining.Results Compared with sham group,the NeuN positive cells were not significantly changed in sham plus stress group （P0.05）;compared with sham group or sham plus stress group,the NeuN positive cells were significantly decreased in ischemia plus stress group （P0.05）;compared with ischemia plus stress group,the NeuN positive cells were not significantly changed in venlafaxine plus ischemia plus stress group（P0.05）;compared with ischemia plus stress group,the NeuN positive cells were significantly increased in quetiapine plus ischemia plus stress group （P0.05）.Compared with sham group,the Nissle staining positive cells were not significantly changed in sham plus stress group （P0.05）;compared with sham group or sham plus stress group, the Nissle staining positive cells were significantly decreased in ischemia plus stress group （P0.05）;compared with ischemia plus stress group,the Nissle staining positive cells were not significantly changed in venlafaxine plus ischemia plus stress group（P0.05）;compared with ischemia plus stress group,the Nissle staining positive cells were significantly increased in quetiapine plus ischemia plus stress group （P0.05）.Conclusion Quetiapine can attenuate the neuronal inju

关 键 词：喹硫平 万拉法新 神经元损伤 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R971[医药卫生—临床医学]