鼻咽癌中p27^kip1表达及其与临床病理特征相关研究  

Expression of p27^kip1 in nasopharyngeal carcinoma and its association with clinicopathological characteristics

作　　者：李宇清[1] 杨惠玲[2] 

机构地区：[1]广州医学院第二附属医院肿瘤科,510260 [2]中山大学中山医学院病理生理教研室

出　　处：《中华生物医学工程杂志》2010年第4期336-339,共4页Chinese Journal of Biomedical Engineering

摘　　要：目的检测p27^kip1蛋白在鼻咽癌中的表达，分析其与临床病理特征的关系。方法收集60例手术活检切取的鼻咽癌组织蜡块，与30例非肿瘤鼻咽组织石蜡标本作比较。应用免疫组织化学技术检测鼻咽癌组织中p27^kip1蛋白的表达，回顾性研究鼻咽癌患者的临床病理特征。结果p27^kip1蛋白在鼻咽癌细胞核和细胞质中均有表达。鼻咽癌组织中细胞核表达阳性率为46．7％（28／60），低于非肿瘤鼻咽组织细胞核表达的90％（27／30），P〈0．01；而在细胞质中阳性率为68．3％（41／60），显著高于非肿瘤鼻咽组织的细胞质表达的20％（6／30），P〈0．01。未发现p27^kip1蛋白在细胞核和细胞质表达与鼻咽癌原发灶的范围和局部侵犯程度有关，但p27^kip1蛋白胞核表达与淋巴结转移、远处转移和TNM临床分期有关；细胞质表达仅与淋巴结转移和TNM临床分期有关，可见p27^kip1蛋白胞核表达与临床病理的关系更为密切。结论与非肿瘤鼻咽组织相比，p27^kip1蛋白为细胞核低表达、细胞质高表达。鼻咽癌中p27^kip1蛋白存在不同于非肿瘤鼻咽组织的错误的核质定位，其在细胞核中表达的减少或丢失可能与鼻咽癌的恶性发展有关，提示p27^kip1蛋白的异常表达和定位可能涉及鼻咽癌的分期和转移。Objective To inrestigate the expression of p27^kip1 protein and its association with clinicopathologic features in nasopharyngeal carcinoma. Methods Immunohistochemistry was performed to detect p27^kip1 expression in 60 paraffin- embedded nasopharyngeal carcinoma samples and 30 paraffin- embedded non-cancer nasopharyngeal tissue samples. Results Nuclear and cytoplasmic p27^kip1 staining was detected in nasopharyngeal carcinoma samples. Of 60 nasopharyngeal carcinoma samples, 46.7% （28/60） were positive for p27^kip1 in cell nuclei, compared with as found in non-cancer nasopharyngeal tissue [90% （27/30） , P〈0.01 ] , and 68.3% （41/60） were positive in cytoplasma, compared with 20% （6/30） of non- cancer nasopharyngeal tissue （P〈0.01）. Both nuclear and cytoplasmic expression of p27^kip1 were not associated to tumor size and local invasion, while positive nuclear expression of p27^kip1 was significantly associated to nodal and remote metastasis and TNM stage. Cytoplasmic expression of p27^kip1 was associated to nodal infiltration and TNM stage. Conclusions Compared to that in non- cancer tissue, p27^kip1 is upregulated in cytoplasm and downregulated in nuclei of nasopharyngeal carcinoma tissue, presenting a mislocalization. Less or no nuclear expression of p27^kip1 may be associated to malignant progression of nasopharyngeal carcinoma. Abnormal expression and location of p27^kip1 may be associated to nasopharyngeal stage and metastasis.

关 键 词：鼻咽癌 免疫组织化学 基因表达 肿瘤标记 生物学 P27^KIP1蛋白 

分 类 号：R739.63[医药卫生—肿瘤]