Livin基因沉默对大肠癌细胞凋亡和化疗敏感性的影响  

Effects of Livin gene silencing on chemotherapeutic sensitivity and apoptosis of colorectal carcinoma cells

作　　者：严红[1] 彭淑梅[1] 罗其泰[1] 邱春雷[1] 延卫东[1] 

机构地区：[1]井冈山大学医学院内科教研室,江西吉安343000

出　　处：《中国老年学杂志》2010年第22期3287-3290,共4页Chinese Journal of Gerontology

基　　金：2010年江西省教育厅科技计划项目青年项目(GJJ10204);2009-2010年度江西省卫生厅科技计划项目(20092099)

摘　　要：目的观察沉默Livin基因对大肠癌HT-29细胞凋亡和化疗敏感性的影响。方法采用脂质体转染技术将化学合成的Livin siRNA转入HT-29细胞,RT-PCR和Western印迹法检测转染效果。选用5-氟尿嘧啶(5-FU)作用于转染和未转染的细胞,MTT检测各组细胞的增殖、流式细胞仪检测细胞的凋亡。结果 si-Livin1转染组LivinαmRNA、LivinβmRNA和Livin蛋白表达水平较其余干扰组和对照组显著降低(P<0.01)。5-FU对HT-29细胞的生长抑制作用强于si-Livin1转染组(P<0.01),而si-Livin1+5-FU组对细胞的生长抑制率显著高于单独si-Livin1转染组和单独5-FU作用组(P<0.01)。与空白对照组比较,si-Livin1转染可使细胞出现明显凋亡现象(P<0.01),5-FU对细胞的促凋亡作用强于si-Livin1转染组(P<0.01),si-Livin1+5-FU组的凋亡率显著高于单独si-Livin1转染组和单独5-FU作用组(P<0.01)。结论 siRNA沉默Livin基因能够抑制HT-29细胞的生长,促其凋亡,提高5-FU的化疗敏感性。Livin基因有望成为大肠癌治疗的新靶点。Objective To explore the effects of Livin gene silencing on chemotherapeutic sensitivity and apoptosis of colorectal carcinoma HT-29 cells.Methods Livin specific siRNA oligonucleotides were designed and synthesized artificially.SiRNA was transfected into colorectal carcinoma HT-29 cells by lipofection technology.Transfection efficiency was detected by Western blot and RT-PCR.The transfected and untransfected cells were incubated in the presence of 5-FU.Cellular proliferation activeties were assayed by MTT.The apoptosis of cells was assayed by flow cytometry（FCM）.Results Compared with those of other transfected groups and control group,the protein levels of Livin and the mRNA expressions of Livinα and Livinβ were decreased significantly after transfected by si-Livin1（P〈0.01）.The growth inhibition rates of cells incubated in 5-FU were higher than those of transfected by si-Livin1（P〈0.01）.The growth inhibition rates of cells affected by si-Livin1 and 5-FU were higher than those of single transfected by si-Livin1 and single incubated in 5-FU significantly（P〈0.01）.The apoptosis rates of cells transfected by si-Livin1 were higher than those of control group （P〈0.01）,but lower than those of incubated in 5-FU（P〈0.01）.The apoptosis rates of cells affected by si-Livin1 and 5-FU were higher than those of single transfected by si-Livin1 and single incubated in 5-FU significantly（P〈0.01）.Conclusions Livin gene silenced by siRNA induces growth suppression and apoptosis of HT-29 cells,which could increase the chemotherapeutic sensitivity of HT-29 cells.Livin gene stands a chance as a new target for colorectal carcinoma treatment.

关 键 词：LIVIN 大肠癌 凋亡抑制蛋白 RNA干扰 细胞凋亡 化疗敏感性 

分 类 号：R735.3[医药卫生—肿瘤]