斑马鱼肠道细菌的分离与生理生化特性  

Isolation of Intestinal Bacteria from Zebrafish (Danio rerio) and Their Physiological and Biochemical characteristics

作　　者：吕爱军[1] 杨正行[1] 刘欢[1] 胡秀彩[2] 张艳华[1] 程超[1] 

机构地区：[1]徐州师范大学生命科学学院,江苏徐州221116 [2]江苏省药用植物生物技术重点实验室,江苏徐州221116

出　　处：《中国农学通报》2010年第24期412-415,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"嗜水气单胞菌诱导斑马鱼皮肤免疫应答的分子机制"(30800847);江苏省高校自然科学基金"草鱼抗菌肽piscidin基因的克隆及表达研究"(08KJD240002;江苏省"青蓝工程""鱼类抗病基因的克隆及其分子机理研究"(2008)

摘　　要：为研究鱼类肠道细菌的分布情况,从模式生物斑马鱼肠道中分离纯化出4株革兰氏阳性菌,暂编号为ZF-1、ZF-2、ZF-3、ZF-4,并进行了菌体形态学观察、生理生化特性等方面的研究。结果表明,分离菌ZF-1株为球菌、ZF-2、、ZF-3、ZF-4株为杆菌、ZF-3株产芽孢;ZF-1和ZF-2株在普通固体培养基上形成圆形、表面湿润、边缘整齐的小菌落,而ZF-3和ZF-4株形成表面干燥、皱褶、边缘不整齐的大菌落;4株菌株均属发酵型代谢、无动力,葡萄糖酸盐、枸橼酸盐、山梨醇、硫化氢、吲哚试验为阴性,β-半乳糖苷酶、苯丙氨酸脱氨酶、七叶苷水解试验为阳性;其中ZF-1株能利用葡萄糖产气、棉子糖、木糖,ZF-2、ZF-4菌株则不能;ZF-3株能发酵侧金盏花醇、不能还原硝酸盐和液化明胶,其他菌株则特性相反。以上结果为进一步鉴定斑马鱼肠道中细菌奠定了基础。The bacteria were isolated from the intestine of zebrafish （Danio rerio）, the four isolated strains were named ZF-1, ZF-2, ZF-3, ZF-4 , respectively. Microscopic examination and bacterial culture results showed that all bacterial isolates were Gram-positive spherical （coccus） or rod-shaped （bacillus） bacteria. The isolates ZF-1 and ZF-2 were formed with the small, round, moist, neat colonies on ordinary nutrient agar plates, while the isolates ZF-3 and ZF-4 were showed with the big, dry, wrinkled surface and irregular colonies. The results of physiological and biochemical test revealed that four isolates were fermentation and nonmotile bacteria, the gluconate, citrate, sorbitol, hydrogen sulfide and indole test were negative, but β-galactosidase, phenylalanine deaminase, nitrogen monoxide and glycosides hydrolysis were positive. Isolated strain ZF-1 could use glucose to produce acid and gas and ferments sugars including raffinose and xylose, while ZF-2 and ZF-4 strains could not use. Isolated strain ZF-3 was positive for adonitol fermentation and negative for nitrate reduction or gelatin liquefaction, while other isolates were reversed. The above results provide a reference for the further identification of intestinal bacteria from zebrafish.

关 键 词：斑马鱼 肠道细菌 分离 生理生化特性 

分 类 号：S917.1[农业科学—水产科学]