正常前列腺不同区带及癌组织来源原代基质细胞的生物学特性及其对前列腺癌细胞株C4-2B的作用  

Different biological characteristics of different prostatic zonal primary stromal cells and cancer associated stromal cells,as well as their different effects on prostate cancer cell line C4-2B

作　　者：王小海[1] 韩邦旻[1] 赵福军[1] 蒋琪[1] 王永传[1] 夏术阶[1] 

机构地区：[1]上海交通大学附属第一人民医院泌尿外科,200080

出　　处：《中华实验外科杂志》2010年第12期1774-1777,共4页Chinese Journal of Experimental Surgery

基　　金：上海市科委重点资助项目（074119518）;上海市科委资助项目（07XD14027）

摘　　要：目的 比较正常前列腺外周带来源原代基质细胞（NPPF）,移行带来源原代基质细胞（NPTF）及前列腺癌组织来源原代基质细胞（CAF）的生物学特性差异,以及它们对前列腺癌细胞株C4-2B的不同影响.方法 苏木素-伊红（HE）染色鉴定前列腺不同区带及癌组织的组织学特征差异;原代培养NPPF、NPTF、CAF,免疫细胞化学染色观察其波形蛋白（Vimentin）、平滑肌动蛋白（SMA）及前列腺特异性抗原（PSA）的表达,生长曲线比较其增殖能力,流式细胞仪检测比较其凋亡率,透射电镜观察比较其超微结构;建立不同来源原代基质细胞与C4-2B细胞株共培养系统,比较不同来源基质细胞对C4-2B细胞增殖（MTT）和凋亡（FCM）方面的影响.结果 NPPF、NPTF、CAF的生长、凋亡、超微结构及对C4-2B细胞的生物学影响存在明显差异,其生长速度依次递增,凋亡率分别为（9.25±2.24）%、（5.98±0.74）%、（2.63±0.96）%,透射电镜提示CAF蛋白质合成最旺盛;C4-2B细胞与基质细胞体外共培养后出现增殖加速,凋亡减少,MTT结果显示共培养2 d后吸光度分别0.540、0.471、0.632,共培养4 d后吸光度分别为0.554、0.488、0.670,P均〈0.05;FCM结果显示与对照组（10.32±0.43）%比较,CAF对C4-2B细胞凋亡的抑制能力最强（3.36±0.17）%,其次是NPPF（5.97±0.70）%和NPTF（8.01±0.22）%,P〈0.05.结论 NPPF、NPTF、CAF的生物学特性存在明显差异,这种差异可能是导致前列腺增生或前列腺癌发生发展的重要原因.Objective To compare the different biological characteristics of normal prostatic peripheral zonal primary stromal cells （normal prostatic peripheral fibroblasts,NPPF）,transition zonal primary stromal cells （normal prostatic transitional fibroblasts,NPTF） and prostate cancer associated primary stromal cells （cancer associated fibroblasts,CAF）,as well as their different effects on prostate cancer cell line C4-2B.Methods HE staining was done to identify the different histological characteristics of prostatic different zonal tissue and cancer tissue.NPPF,NPTF and CAF were primarily cultured,and the expression of Vimentin,smooth muscle actin （SMA） and prostatic specific antigen （PSA） was detected by using immunocytochemistry.Their proliferation was compared by growth curves,apoptosis rate by flow cytometry （FCM）,and cellular ultrastructure by transmission electron microscopy.The co-culture system of primary stromal cells and C4-2B cells were established,and the changes in proliferation and apoptosis of C4-2B cells affected by different stromal cell subsets were compared.Results There was significant difference in proliferation,apoptosis,ultrastructure among NPPF,NPTF and CAF,and their biological effects on C4-2B cells.Growth rate of NPPF,NPTF and CAF was increased in turn,but their apoptosis decreased in turn [9.25 ± 2.24）%,（5.98 ± 0.74）%,（2.63 ± 0.96）%,respectively].Transmission electron microscopy suggested that CAF had the most productive protein synthesis.After C4-2B cells were co-cultured with the prostate stromal cell subsets,proliferation was increased,but apoptosis was decreased.Methylthiazol tetrazolium （MTT） assay showed that the absorbance in C4-2B cells after co-culture with NPPF,NPTF and CAF for 2 and 4 days was 0.540,0.471,0.632,and 0.554,0.488,0.670,respectively,P 〈0.05.FCM revealed that as compared with the control group （10.32 ±0.43）%,CAF had the strongest capability to inhibit the apoptosis of C4-2B cells （3.36±0.17）%,followed by NPPF （5.97 ±0.

关 键 词：前列腺癌 移行带 基质细胞 共培养 

分 类 号：R737.25[医药卫生—肿瘤]