成体神经干细胞脑内移植对脑外伤大鼠神经功能的影响  

Effect of adult neural stem cell intracerebral implantation on neurological function of rats with traumatic brain injury

作　　者：徐国政[1] 刁波[2] 张宜[2] 文雪[3] 袁先厚[1] 

机构地区：[1]武汉大学中南医院神经外科,430070 [2]广州军区武汉总医院医学实验科 [3]广州军区武汉总医院神经外科

出　　处：《中华实验外科杂志》2010年第12期1907-1909,共3页Chinese Journal of Experimental Surgery

摘　　要：目的 观察脑内移植成体神经干细胞对脑外伤大鼠中枢神经功能的影响.方法 将60只Wistar大鼠随机分为5组,正常组（A）、模型组（B）、假手术组（C）、PBS移植组（D）、干细胞移植组（E）.Morris水迷宫实验测试各组大鼠的学习记忆能力;免疫组织化学及酶联免疫吸附试验（ELISA）检测大鼠脑组织中脑源性神经生长因子（BDNF）、神经生长因子（NGF）的表达.结果与A组比较,B组大鼠寻找隐形平台的时间明显延长（P〈0.01）,脑组织中BDNF（27.70±1.65）ng/g蛋白、NGF（59.80±9.40）ng/g蛋白含量下降显著（P〈0.01）,模型复制效果理想;与B组比较,E组大鼠学习及功能明显改善,脑内BDNF（49.30±2.87）ng/g蛋白、NGF（88.50±9.23）ng/g蛋白的表达量上升,差异有统计学意义（P〈0.01）.结论 成体神经干细胞的脑内移植能明显恢复外伤大鼠中枢神经功能.Objective To study the neuroprotetive effect of dault neural stem cells intracerebral implantation to rats subjected to traumatic brain injury.Methods Sixty Wistar rats were divided into 5 groups randomly： normal group （ A ）,model group （ B）,sham-operated group （ C ）,PBS transplated group （D）,neural stem cells transplated group （E）.Morris maze method was adopted to observe the impact on the learning and memory.Immunohistochemisty and enzyme linked immunosorbent assay （ELISA）were used to detect the expression of brain derived neurotrophic factor （BDNF） and nerve growth factor （NGF） in brain issue.Results As compared with group A,in Morris maze experiment,time to reach the end point of the maze in group B was significantly prolonged （ P 〈 0.01 ）,and the content of BDNF （27.70 ± 1.65 ） ng/g prot and NGF （ 59.80 ± 9.40 ） ng/g prot were decreased significantly （ P 〈 0.01 ）.As compared with group B,the function of learning and memory was obviously improved,and the expression of BDNF （49.30 ±2.87） ng/g prot and NGF （88.50 ±9.23） ng/gprot in brain issues were statistically increased in group E （P 〈0.01 ）.Conclusion Transplantation of adult neural stem cells can ameliorate learning and memory deficits following traumatic brain injury in rats.

关 键 词：成体神经干细胞 脑内移植 脑外伤 

分 类 号：R651.1[医药卫生—外科学]